Maturation-promoting factor and p34cdc2 kinase during oocyte maturation of the Japanese quail

M. Mori, M. Yamashita, M. Yoshikuni, S. Fukada, Y. Nagahama

研究成果: Contribution to journalArticle査読

11 被引用数 (Scopus)

抄録

Maturation-promoting factor and a homolog of fission yeast cdc2+ gene product (p34cdc2) were investigated during the final 24 hr of maturation of quail oocytes. Kinase activity of p34cdc2 in the oocyte germinal disk (GD) increased 15 times at maturation. Two bands, at 32 and 34 kDa, were detected in immature oocytes by immunoblotting of SDS-PAGE with anti-p34cdc2 monoclonal antibody. A new band, which is close to the 32-kDa band but with a slightly faster mobility, appeared during maturation. No p34cdc2 could be detected outside the GD. Microinjection of GD extract from mature oocytes caused maturation of Xenopus oocytes.

本文言語英語
ページ(範囲)246-249
ページ数4
ジャーナルDevelopmental Biology
146
1
DOI
出版ステータス出版済み - 7 1991
外部発表はい

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Developmental Biology
  • Cell Biology

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