Measurement and correlation of phase equilibria of aqueous two- phase systems and partition coefficients of enzymes

Aqueous two-phase systems provide a successful method for separating biomolecules by extraction under mild condition. Nevertheless, the phase equilibrium data of aqueous two-phase systems and the partition coefficient data of biomolecules are scarce and it is difficult to correlate them quantitatively. The phase equilibria for the dextran-polyethylene glycol-water aqueous two-phase systems are measured. The partition coefficients of enzymes are also measured. Further, the osmotic virial equation is used to correlate the phase equilibria of the aqueous two-phase systems and the partition coefficients of enzymes. (from Authors)