Measurement of adenylyl cyclase by separating cyclic AMP on silica gel thin-layer chromatography

Haruhiro Higashida, Kazi Z. Hossain, Hiroko Takahagi, Mami Noda

研究成果: Contribution to journalArticle査読

11 被引用数 (Scopus)

抄録

A procedure for isolation of cyclic AMP (cAMP) by thin-layer chromatography on silica gel is described. One-dimensional ascending chromatograms were developed using [H2O/C2H5OH/NH4HCO3 (30%:70%:0.2 M)] as the mobile phase. This procedure separated [32P]cAMP from other radioactive metabolites of [32P]ATP in up to 19 samples on one sheet (20 × 10cm) over 40-60 min at room temperature (21 °C). This simple and rapid isolation method provides a novel and convenient technique for the assay of adenylyl cyclase.

本文言語英語
ページ(範囲)106-111
ページ数6
ジャーナルAnalytical Biochemistry
308
1
DOI
出版ステータス出版済み - 9 1 2002

All Science Journal Classification (ASJC) codes

  • 生物理学
  • 生化学
  • 分子生物学
  • 細胞生物学

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