Mechanism for the antibacterial action of epigallocatechin gallate (EGCg) on Bacillus subtilis

Motokazu Nakayama, Kanami Shimatani, Tadahiro Ozawa, Naofumi Shigemune, Daisuke Tomiyama, Koji Yui, Mao Katsuki, Keisuke Ikeda, Ai Nonaka, Takahisa Miyamoto

研究成果: Contribution to journalArticle査読

38 被引用数 (Scopus)

抄録

Catechins are a class of polyphenols and have high anti-bacterial activity against various microorganisms. Here, we report the mechanism for antibacterial activity of epigallocatechin gallate (EGCg) against Gram-positive bacteria Bacillus subtilis, which is highly sensitive to EGCg. Transmission electron microscope analysis revealed that deposits containing EGCg were found throughout the cell envelope from the outermost surface to the outer surface of cytoplasmic membrane. Aggregating forms of proteins and EGCg were identified as spots that disappeared or showed markedly decreased intensity after the treatment with EGCg compared to the control by two-dimensional electrophoresis. Among the identified proteins included 4 cell surface proteins, such as oligopeptide ABC transporter binding lipoprotein, glucose phosphotransferase system transporter protein, phosphate ABC transporter substrate-binding protein, and penicillin- binding protein 5. Observations of glucose uptake of cells and cell shape B. subtilis after the treatment with EGCg suggested that EGCg inhibits the major functions of these proteins, leading to growth inhibition of B. subtilis.

本文言語英語
ページ(範囲)845-854
ページ数10
ジャーナルBioscience, Biotechnology and Biochemistry
79
5
DOI
出版ステータス出版済み - 1 1 2015

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

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