Mechanism of Ca2+ increase in myoblasts derived from chicken embryos

Shoji Tabata, Yoh Takemura, Masaaki Kobayashi, Masachika Ikeda, Shotaro Nishimura, Yusuke Sato, Ryuichi Tatsumi, Yoshihide Ikeuchi, Hisao Iwamoto

研究成果: ジャーナルへの寄稿記事

2 引用 (Scopus)

抄録

The mechanism of intracellular calcium ions (Ca2+) increase in chicken myoblasts was studied using histological, immunohistochemical, immunoblotting and Ca2+ imaging techniques. Mononuclear myoblasts at embryonic day 12 (E12) contained myofibrils in the peripheral cytoplasm, and the sarcoplasmic reticulum was observed in the cytoplasm. Several Ca 2+-related receptors, namely acetylcholine (ACh) receptors, dihydropyridine receptors (DHPRs) and ryanodine receptors (RyRs), were detected in the tissue as early as E12. Western blotting analyses detected one band corresponding to RyR subtype 3 (RyR3) at E12 and two bands corresponding to RyR1 and RyR3 after E13. Ca2+ imaging of mononuclear myoblasts in vitro revealed an intense Ca2+-increase response to ACh stimulation, and this effect was abolished after EGTA addition to the culture medium. Nifedipine treatment also led to a lack of Ca2+ increase in response to ACh stimulation, while ryanodine treatment led to a weak Ca2+-increase response. On the other hand, multinuclear myoblasts showed a Ca 2+-increase response to ACh stimulation in the presence of not only EGTA but also nifedipine, although ryanodine treatment led to a lack of Ca 2+ increase. These results suggest that the mechanism of Ca 2+ increase in mononuclear myoblasts involves extracellular Ca 2+ entry through DHPRs, which is amplified by Ca2+ release from the cytoplasmic Ca2+ store, while multinuclear myoblasts mainly depend on Ca2+ release from the cytoplasmic Ca2+ store.

元の言語英語
ページ(範囲)265-271
ページ数7
ジャーナルJournal of Electron Microscopy
55
発行部数5
DOI
出版物ステータス出版済み - 10 1 2006

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chickens
Myoblasts
embryos
acetylcholine
Chickens
Embryonic Structures
Imaging techniques
stimulation
cytoplasm
Acetylcholine
Calcium
Ryanodine
L-Type Calcium Channels
Ryanodine Receptor Calcium Release Channel
Egtazic Acid
Nifedipine
Tissue
sarcoplasmic reticulum
Ions
Cytoplasm

All Science Journal Classification (ASJC) codes

  • Instrumentation

これを引用

Mechanism of Ca2+ increase in myoblasts derived from chicken embryos. / Tabata, Shoji; Takemura, Yoh; Kobayashi, Masaaki; Ikeda, Masachika; Nishimura, Shotaro; Sato, Yusuke; Tatsumi, Ryuichi; Ikeuchi, Yoshihide; Iwamoto, Hisao.

:: Journal of Electron Microscopy, 巻 55, 番号 5, 01.10.2006, p. 265-271.

研究成果: ジャーナルへの寄稿記事

Tabata, S, Takemura, Y, Kobayashi, M, Ikeda, M, Nishimura, S, Sato, Y, Tatsumi, R, Ikeuchi, Y & Iwamoto, H 2006, 'Mechanism of Ca2+ increase in myoblasts derived from chicken embryos', Journal of Electron Microscopy, 巻. 55, 番号 5, pp. 265-271. https://doi.org/10.1093/jmicro/dfl033
Tabata, Shoji ; Takemura, Yoh ; Kobayashi, Masaaki ; Ikeda, Masachika ; Nishimura, Shotaro ; Sato, Yusuke ; Tatsumi, Ryuichi ; Ikeuchi, Yoshihide ; Iwamoto, Hisao. / Mechanism of Ca2+ increase in myoblasts derived from chicken embryos. :: Journal of Electron Microscopy. 2006 ; 巻 55, 番号 5. pp. 265-271.
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abstract = "The mechanism of intracellular calcium ions (Ca2+) increase in chicken myoblasts was studied using histological, immunohistochemical, immunoblotting and Ca2+ imaging techniques. Mononuclear myoblasts at embryonic day 12 (E12) contained myofibrils in the peripheral cytoplasm, and the sarcoplasmic reticulum was observed in the cytoplasm. Several Ca 2+-related receptors, namely acetylcholine (ACh) receptors, dihydropyridine receptors (DHPRs) and ryanodine receptors (RyRs), were detected in the tissue as early as E12. Western blotting analyses detected one band corresponding to RyR subtype 3 (RyR3) at E12 and two bands corresponding to RyR1 and RyR3 after E13. Ca2+ imaging of mononuclear myoblasts in vitro revealed an intense Ca2+-increase response to ACh stimulation, and this effect was abolished after EGTA addition to the culture medium. Nifedipine treatment also led to a lack of Ca2+ increase in response to ACh stimulation, while ryanodine treatment led to a weak Ca2+-increase response. On the other hand, multinuclear myoblasts showed a Ca 2+-increase response to ACh stimulation in the presence of not only EGTA but also nifedipine, although ryanodine treatment led to a lack of Ca 2+ increase. These results suggest that the mechanism of Ca 2+ increase in mononuclear myoblasts involves extracellular Ca 2+ entry through DHPRs, which is amplified by Ca2+ release from the cytoplasmic Ca2+ store, while multinuclear myoblasts mainly depend on Ca2+ release from the cytoplasmic Ca2+ store.",
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AU - Tabata, Shoji

AU - Takemura, Yoh

AU - Kobayashi, Masaaki

AU - Ikeda, Masachika

AU - Nishimura, Shotaro

AU - Sato, Yusuke

AU - Tatsumi, Ryuichi

AU - Ikeuchi, Yoshihide

AU - Iwamoto, Hisao

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N2 - The mechanism of intracellular calcium ions (Ca2+) increase in chicken myoblasts was studied using histological, immunohistochemical, immunoblotting and Ca2+ imaging techniques. Mononuclear myoblasts at embryonic day 12 (E12) contained myofibrils in the peripheral cytoplasm, and the sarcoplasmic reticulum was observed in the cytoplasm. Several Ca 2+-related receptors, namely acetylcholine (ACh) receptors, dihydropyridine receptors (DHPRs) and ryanodine receptors (RyRs), were detected in the tissue as early as E12. Western blotting analyses detected one band corresponding to RyR subtype 3 (RyR3) at E12 and two bands corresponding to RyR1 and RyR3 after E13. Ca2+ imaging of mononuclear myoblasts in vitro revealed an intense Ca2+-increase response to ACh stimulation, and this effect was abolished after EGTA addition to the culture medium. Nifedipine treatment also led to a lack of Ca2+ increase in response to ACh stimulation, while ryanodine treatment led to a weak Ca2+-increase response. On the other hand, multinuclear myoblasts showed a Ca 2+-increase response to ACh stimulation in the presence of not only EGTA but also nifedipine, although ryanodine treatment led to a lack of Ca 2+ increase. These results suggest that the mechanism of Ca 2+ increase in mononuclear myoblasts involves extracellular Ca 2+ entry through DHPRs, which is amplified by Ca2+ release from the cytoplasmic Ca2+ store, while multinuclear myoblasts mainly depend on Ca2+ release from the cytoplasmic Ca2+ store.

AB - The mechanism of intracellular calcium ions (Ca2+) increase in chicken myoblasts was studied using histological, immunohistochemical, immunoblotting and Ca2+ imaging techniques. Mononuclear myoblasts at embryonic day 12 (E12) contained myofibrils in the peripheral cytoplasm, and the sarcoplasmic reticulum was observed in the cytoplasm. Several Ca 2+-related receptors, namely acetylcholine (ACh) receptors, dihydropyridine receptors (DHPRs) and ryanodine receptors (RyRs), were detected in the tissue as early as E12. Western blotting analyses detected one band corresponding to RyR subtype 3 (RyR3) at E12 and two bands corresponding to RyR1 and RyR3 after E13. Ca2+ imaging of mononuclear myoblasts in vitro revealed an intense Ca2+-increase response to ACh stimulation, and this effect was abolished after EGTA addition to the culture medium. Nifedipine treatment also led to a lack of Ca2+ increase in response to ACh stimulation, while ryanodine treatment led to a weak Ca2+-increase response. On the other hand, multinuclear myoblasts showed a Ca 2+-increase response to ACh stimulation in the presence of not only EGTA but also nifedipine, although ryanodine treatment led to a lack of Ca 2+ increase. These results suggest that the mechanism of Ca 2+ increase in mononuclear myoblasts involves extracellular Ca 2+ entry through DHPRs, which is amplified by Ca2+ release from the cytoplasmic Ca2+ store, while multinuclear myoblasts mainly depend on Ca2+ release from the cytoplasmic Ca2+ store.

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