Mechanism of murine Vγ1+ γδ T Cell-mediated innate immune response against Listeria monocytogenes infection

Goro Matsuzaki, Hisakata Yamada, Kenji Kishihara, Yasunobu Yoshikai, Kikuo Nomoto

研究成果: Contribution to journalArticle査読

36 被引用数 (Scopus)

抄録

Murine γδ T cells participate in innate immune response against infection of the intracellular bacterium Listeria monocytogenes. In the present report, we analyzed the mechanism of the γδ T cell-mediated response against L. monocytogenes infection. γδ T cell-enriched spleen cells of L. monocytogenes-infected mice produced IFN-γ in vitro in response to L. monocytogenes-infected spleen cells. The IFN-γ production was abrogated by depletion of Vγ1+ γδ T cells. IFN-γ production of the Vγ1+ γδ T cells in response to L. monocytogenes-infected spleen cells required IL-12. However, addition of Fab fragment of anti-TCR γδ monoclonal antibodies (mAb) failed to block the response, suggesting that the response requires no TCR-mediated antigen recognition. Interestingly, Vγ1+ γδ T cells of naive mice also produced IFN-γ in response to L. monocytogenes-infected spleen cells in an IL-12-dependent manner. Furthermore, the IL-12 receptor (IL-12R) gene was expressed on the Vγ1+ γδ T cells of naive mice as well as those of L. monocytogenes-infected mice although naive αβ T cells lack IL-12R expression. All the results suggest that the Vγ1+ γδ T cells participate in immune surveillance against intracellular bacterial infection through quick production of IFN-γ in response to infection-induced IL-12 without antigen-driven clonal expansion and maturation.

本文言語英語
ページ(範囲)928-935
ページ数8
ジャーナルEuropean Journal of Immunology
32
4
DOI
出版ステータス出版済み - 2002

All Science Journal Classification (ASJC) codes

  • 免疫アレルギー学
  • 免疫学

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