Mechanisms involved in suppression of NGF-induced neuronal differentiation of PC12 cells by hyaluronic acid

Ayako Washio, Chiaki Kitamura, Eijiro Jimi, Masamichi Terashita, Tatsuji Nishihara

研究成果: ジャーナルへの寄稿記事

21 引用 (Scopus)

抄録

In the present study, we found that hyaluronic acid (HA) suppressed the neuronal differentiation mediated by nerve growth factor (NGF). In addition, we examined the mechanism by which HA inhibits the NGF-induced neurite outgrowth of PC12 cells. We elucidated the direct interaction between NGF and HA, and found that HA did not bind to NGF directly using a quartz-crystal microbalance. Western blot analysis revealed that HA suppressed NGF-induced phosphorylation of p38 MAPK, ERKs, and transcriptional factor CREB in PC12 cells. Furthermore, HA inhibited the luciferase activity of pCRE-Luc transfected PC12 cells in the presence of NGF. We confirmed that the p38 MAPK inhibitor SB203580 and ERK inhibitor U0126 suppressed NGF-induced neurite outgrowth of PC12 cells, and found that the inhibitory effects of HA on phosphorylation of ERKs, but not of p38 MAPK, were restored by the anti-RHAMM antibody. The number of PC12 cells with neurites increased remarkably when pre-cultured with the anti-RHAMM antibody, then treated with NGF and HA. Our findings indicate that HA inhibits NGF-induced neuronal differentiation of PC12 cells partially by inhibiting ERK phosphorylation through RHAMM, and suggest that the binding of HA to RHAMM modifies the signaling pathways in PC12 cells treated with NGF.

元の言語英語
ページ(範囲)3036-3043
ページ数8
ジャーナルExperimental Cell Research
315
発行部数17
DOI
出版物ステータス出版済み - 10 15 2009
外部発表Yes

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PC12 Cells
Nerve Growth Factor
Hyaluronic Acid
p38 Mitogen-Activated Protein Kinases
Phosphorylation
Anti-Idiotypic Antibodies
Quartz Crystal Microbalance Techniques
Neurites
Luciferases
Western Blotting

All Science Journal Classification (ASJC) codes

  • Cell Biology

これを引用

Mechanisms involved in suppression of NGF-induced neuronal differentiation of PC12 cells by hyaluronic acid. / Washio, Ayako; Kitamura, Chiaki; Jimi, Eijiro; Terashita, Masamichi; Nishihara, Tatsuji.

:: Experimental Cell Research, 巻 315, 番号 17, 15.10.2009, p. 3036-3043.

研究成果: ジャーナルへの寄稿記事

Washio, Ayako ; Kitamura, Chiaki ; Jimi, Eijiro ; Terashita, Masamichi ; Nishihara, Tatsuji. / Mechanisms involved in suppression of NGF-induced neuronal differentiation of PC12 cells by hyaluronic acid. :: Experimental Cell Research. 2009 ; 巻 315, 番号 17. pp. 3036-3043.
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