Mediator cyclin-dependent kinases upregulate transcription of inflammatory genes in cooperation with NF-κB and C/EBPβ on stimulation of Toll-like receptor 9

Seiji Yamamoto, Tomoko Hagihara, Yoshiyuki Horiuchi, Akira Okui, Shotaro Wani, Tokuyuki Yoshida, Takao Inoue, Aki Tanaka, Takashi Ito, Yutaka Hirose, Yoshiaki Ohkuma

研究成果: ジャーナルへの寄稿記事

6 引用 (Scopus)

抄録

In eukaryotes, the Mediator complex has important roles in regulation of transcription by RNA polymerase II. Mediator is a large complex with more than 20 subunits that form head, middle, tail and CDK/cyclin modules. Among them, CDK8 and/or CDK19 (CDK8/19), and their counterpart cyclin C, form the CDK/cyclin module together with Mediator subunits MED12 and MED13. Despite evidences of both activation and repression, the precise functional roles of CDK8/19 in transcription are still elusive. Our previous results indicate that CDK8/19 recruits epigenetic regulators to repress immunoresponse genes. Here, this study focused on Toll-like receptors (TLRs), which exert innate immune responses through recognition of pathogen-associated molecular patterns and examined the functional roles of CDK8/19. As a result, CDK8/19 regulated transcription of inflammatory genes on stimulation of TLR9 in myeloma-derived RPMI8226 cells, which led to expression of inflammation-associated genes such as IL8, IL10, PTX3 and CCL2. Mediator subunits CDK8/19 and MED1, inflammation-related transcriptional activator NF-κB and C/EBPβ, and general transcription factors TFIIE and TFIIB colocalized at the promoter regions of these genes under this condition. Our results show that CDK8/19 positively regulates inflammatory gene transcription in cooperation with NF-κB and C/EBPβ on stimulation of TLR9.

元の言語英語
ページ(範囲)265-276
ページ数12
ジャーナルGenes to Cells
22
発行部数3
DOI
出版物ステータス出版済み - 3 1 2017

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Toll-Like Receptor 9
Cyclin-Dependent Kinases
Up-Regulation
Cyclins
Genes
Cyclin C
Mediator Complex
Transcription Factor TFIIB
General Transcription Factors
Inflammation
RNA Polymerase II
Toll-Like Receptors
Eukaryota
Interleukin-8
Innate Immunity
Genetic Promoter Regions
Epigenomics
Interleukin-10
Head

All Science Journal Classification (ASJC) codes

  • Genetics
  • Cell Biology

これを引用

Mediator cyclin-dependent kinases upregulate transcription of inflammatory genes in cooperation with NF-κB and C/EBPβ on stimulation of Toll-like receptor 9. / Yamamoto, Seiji; Hagihara, Tomoko; Horiuchi, Yoshiyuki; Okui, Akira; Wani, Shotaro; Yoshida, Tokuyuki; Inoue, Takao; Tanaka, Aki; Ito, Takashi; Hirose, Yutaka; Ohkuma, Yoshiaki.

:: Genes to Cells, 巻 22, 番号 3, 01.03.2017, p. 265-276.

研究成果: ジャーナルへの寄稿記事

Yamamoto, S, Hagihara, T, Horiuchi, Y, Okui, A, Wani, S, Yoshida, T, Inoue, T, Tanaka, A, Ito, T, Hirose, Y & Ohkuma, Y 2017, 'Mediator cyclin-dependent kinases upregulate transcription of inflammatory genes in cooperation with NF-κB and C/EBPβ on stimulation of Toll-like receptor 9', Genes to Cells, 巻. 22, 番号 3, pp. 265-276. https://doi.org/10.1111/gtc.12475
Yamamoto, Seiji ; Hagihara, Tomoko ; Horiuchi, Yoshiyuki ; Okui, Akira ; Wani, Shotaro ; Yoshida, Tokuyuki ; Inoue, Takao ; Tanaka, Aki ; Ito, Takashi ; Hirose, Yutaka ; Ohkuma, Yoshiaki. / Mediator cyclin-dependent kinases upregulate transcription of inflammatory genes in cooperation with NF-κB and C/EBPβ on stimulation of Toll-like receptor 9. :: Genes to Cells. 2017 ; 巻 22, 番号 3. pp. 265-276.
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abstract = "In eukaryotes, the Mediator complex has important roles in regulation of transcription by RNA polymerase II. Mediator is a large complex with more than 20 subunits that form head, middle, tail and CDK/cyclin modules. Among them, CDK8 and/or CDK19 (CDK8/19), and their counterpart cyclin C, form the CDK/cyclin module together with Mediator subunits MED12 and MED13. Despite evidences of both activation and repression, the precise functional roles of CDK8/19 in transcription are still elusive. Our previous results indicate that CDK8/19 recruits epigenetic regulators to repress immunoresponse genes. Here, this study focused on Toll-like receptors (TLRs), which exert innate immune responses through recognition of pathogen-associated molecular patterns and examined the functional roles of CDK8/19. As a result, CDK8/19 regulated transcription of inflammatory genes on stimulation of TLR9 in myeloma-derived RPMI8226 cells, which led to expression of inflammation-associated genes such as IL8, IL10, PTX3 and CCL2. Mediator subunits CDK8/19 and MED1, inflammation-related transcriptional activator NF-κB and C/EBPβ, and general transcription factors TFIIE and TFIIB colocalized at the promoter regions of these genes under this condition. Our results show that CDK8/19 positively regulates inflammatory gene transcription in cooperation with NF-κB and C/EBPβ on stimulation of TLR9.",
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T1 - Mediator cyclin-dependent kinases upregulate transcription of inflammatory genes in cooperation with NF-κB and C/EBPβ on stimulation of Toll-like receptor 9

AU - Yamamoto, Seiji

AU - Hagihara, Tomoko

AU - Horiuchi, Yoshiyuki

AU - Okui, Akira

AU - Wani, Shotaro

AU - Yoshida, Tokuyuki

AU - Inoue, Takao

AU - Tanaka, Aki

AU - Ito, Takashi

AU - Hirose, Yutaka

AU - Ohkuma, Yoshiaki

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