Melanoma-targeted chemo-thermo-immuno (CTI)-therapy using N-propionyl-4-S-cysteaminylphenol-magnetite nanoparticles elicits CTL response via heat shock protein-peptide complex release

Akiko Sato, Yasuaki Tamura, Noriyuki Sato, Toshiharu Yamashita, Tomoaki Takada, Makito Sato, Yasue Osai, Masae Okura, Ichiro Ono, Akira Ito, Hiroyuki Honda, Kazumasa Wakamatsu, Shosuke Ito, Kowichi Jimbow

研究成果: ジャーナルへの寄稿記事

25 引用 (Scopus)

抄録

Melanogenesis substrate, N-propionyl-4-S-cysteaminylphenol (NPrCAP) is specifically taken up by melanoma cells and inhibits their growth by producing cytotxic free radicals. By taking advantage of this unique chemical agent, we have established melanoma-targeting intracellular hyperthermia by conjugating NPrCAP with magnetite nanoparticles (NPrCAP/M) upon exposure to an alternating magnetic field (AMF). This treatment causes cytotoxic reaction as well as heat shock responses, leading to elicitation of antitumor immune response, which was proved by tumor rechallenge test and CTL induction. We found the level of heat shock protein 72 (Hsp72) to be increased in the cell lysate and culture supernatant after intracellular hyperthermia. Melanoma-specific CD8+ T-cell response to dendritic cells loaded with hyperthermia-treated tumor lysate was enhanced when compared with non-treated tumor lysate. When heat shock protein, particularly Hsp72, was immuno-depleted from hyperthermia-treated tumor cell lysate, specific CD8+ T-cell response was abolished. Thus, it is suggested that antitumor immune response induced by hyperthermia using NPrCAP/M is derived from the release of HSP-peptide complex from degraded tumor cells. Therefore, this chemo-thermo-immuno (CTI)-therapy might be effective not only for primary melanoma but also for distant metastasis because of induction of systemic antimelanoma immune responses.

元の言語英語
ページ(範囲)1939-1946
ページ数8
ジャーナルCancer Science
101
発行部数9
DOI
出版物ステータス出版済み - 9 1 2010

Fingerprint

Magnetite Nanoparticles
Heat-Shock Proteins
Melanoma
Fever
Peptides
HSP72 Heat-Shock Proteins
Neoplasms
T-Lymphocytes
Therapeutics
Heat-Shock Response
Induced Hyperthermia
Magnetic Fields
Dendritic Cells
Free Radicals
Cell Culture Techniques
N-propionyl-4-S-cysteaminylphenol
Neoplasm Metastasis
Growth

All Science Journal Classification (ASJC) codes

  • Oncology
  • Cancer Research

これを引用

Melanoma-targeted chemo-thermo-immuno (CTI)-therapy using N-propionyl-4-S-cysteaminylphenol-magnetite nanoparticles elicits CTL response via heat shock protein-peptide complex release. / Sato, Akiko; Tamura, Yasuaki; Sato, Noriyuki; Yamashita, Toshiharu; Takada, Tomoaki; Sato, Makito; Osai, Yasue; Okura, Masae; Ono, Ichiro; Ito, Akira; Honda, Hiroyuki; Wakamatsu, Kazumasa; Ito, Shosuke; Jimbow, Kowichi.

:: Cancer Science, 巻 101, 番号 9, 01.09.2010, p. 1939-1946.

研究成果: ジャーナルへの寄稿記事

Sato, A, Tamura, Y, Sato, N, Yamashita, T, Takada, T, Sato, M, Osai, Y, Okura, M, Ono, I, Ito, A, Honda, H, Wakamatsu, K, Ito, S & Jimbow, K 2010, 'Melanoma-targeted chemo-thermo-immuno (CTI)-therapy using N-propionyl-4-S-cysteaminylphenol-magnetite nanoparticles elicits CTL response via heat shock protein-peptide complex release', Cancer Science, 巻. 101, 番号 9, pp. 1939-1946. https://doi.org/10.1111/j.1349-7006.2010.01623.x
Sato, Akiko ; Tamura, Yasuaki ; Sato, Noriyuki ; Yamashita, Toshiharu ; Takada, Tomoaki ; Sato, Makito ; Osai, Yasue ; Okura, Masae ; Ono, Ichiro ; Ito, Akira ; Honda, Hiroyuki ; Wakamatsu, Kazumasa ; Ito, Shosuke ; Jimbow, Kowichi. / Melanoma-targeted chemo-thermo-immuno (CTI)-therapy using N-propionyl-4-S-cysteaminylphenol-magnetite nanoparticles elicits CTL response via heat shock protein-peptide complex release. :: Cancer Science. 2010 ; 巻 101, 番号 9. pp. 1939-1946.
@article{325213447a314020a51189e7604d51d7,
title = "Melanoma-targeted chemo-thermo-immuno (CTI)-therapy using N-propionyl-4-S-cysteaminylphenol-magnetite nanoparticles elicits CTL response via heat shock protein-peptide complex release",
abstract = "Melanogenesis substrate, N-propionyl-4-S-cysteaminylphenol (NPrCAP) is specifically taken up by melanoma cells and inhibits their growth by producing cytotxic free radicals. By taking advantage of this unique chemical agent, we have established melanoma-targeting intracellular hyperthermia by conjugating NPrCAP with magnetite nanoparticles (NPrCAP/M) upon exposure to an alternating magnetic field (AMF). This treatment causes cytotoxic reaction as well as heat shock responses, leading to elicitation of antitumor immune response, which was proved by tumor rechallenge test and CTL induction. We found the level of heat shock protein 72 (Hsp72) to be increased in the cell lysate and culture supernatant after intracellular hyperthermia. Melanoma-specific CD8+ T-cell response to dendritic cells loaded with hyperthermia-treated tumor lysate was enhanced when compared with non-treated tumor lysate. When heat shock protein, particularly Hsp72, was immuno-depleted from hyperthermia-treated tumor cell lysate, specific CD8+ T-cell response was abolished. Thus, it is suggested that antitumor immune response induced by hyperthermia using NPrCAP/M is derived from the release of HSP-peptide complex from degraded tumor cells. Therefore, this chemo-thermo-immuno (CTI)-therapy might be effective not only for primary melanoma but also for distant metastasis because of induction of systemic antimelanoma immune responses.",
author = "Akiko Sato and Yasuaki Tamura and Noriyuki Sato and Toshiharu Yamashita and Tomoaki Takada and Makito Sato and Yasue Osai and Masae Okura and Ichiro Ono and Akira Ito and Hiroyuki Honda and Kazumasa Wakamatsu and Shosuke Ito and Kowichi Jimbow",
year = "2010",
month = "9",
day = "1",
doi = "10.1111/j.1349-7006.2010.01623.x",
language = "English",
volume = "101",
pages = "1939--1946",
journal = "Cancer Science",
issn = "1347-9032",
publisher = "Wiley-Blackwell",
number = "9",

}

TY - JOUR

T1 - Melanoma-targeted chemo-thermo-immuno (CTI)-therapy using N-propionyl-4-S-cysteaminylphenol-magnetite nanoparticles elicits CTL response via heat shock protein-peptide complex release

AU - Sato, Akiko

AU - Tamura, Yasuaki

AU - Sato, Noriyuki

AU - Yamashita, Toshiharu

AU - Takada, Tomoaki

AU - Sato, Makito

AU - Osai, Yasue

AU - Okura, Masae

AU - Ono, Ichiro

AU - Ito, Akira

AU - Honda, Hiroyuki

AU - Wakamatsu, Kazumasa

AU - Ito, Shosuke

AU - Jimbow, Kowichi

PY - 2010/9/1

Y1 - 2010/9/1

N2 - Melanogenesis substrate, N-propionyl-4-S-cysteaminylphenol (NPrCAP) is specifically taken up by melanoma cells and inhibits their growth by producing cytotxic free radicals. By taking advantage of this unique chemical agent, we have established melanoma-targeting intracellular hyperthermia by conjugating NPrCAP with magnetite nanoparticles (NPrCAP/M) upon exposure to an alternating magnetic field (AMF). This treatment causes cytotoxic reaction as well as heat shock responses, leading to elicitation of antitumor immune response, which was proved by tumor rechallenge test and CTL induction. We found the level of heat shock protein 72 (Hsp72) to be increased in the cell lysate and culture supernatant after intracellular hyperthermia. Melanoma-specific CD8+ T-cell response to dendritic cells loaded with hyperthermia-treated tumor lysate was enhanced when compared with non-treated tumor lysate. When heat shock protein, particularly Hsp72, was immuno-depleted from hyperthermia-treated tumor cell lysate, specific CD8+ T-cell response was abolished. Thus, it is suggested that antitumor immune response induced by hyperthermia using NPrCAP/M is derived from the release of HSP-peptide complex from degraded tumor cells. Therefore, this chemo-thermo-immuno (CTI)-therapy might be effective not only for primary melanoma but also for distant metastasis because of induction of systemic antimelanoma immune responses.

AB - Melanogenesis substrate, N-propionyl-4-S-cysteaminylphenol (NPrCAP) is specifically taken up by melanoma cells and inhibits their growth by producing cytotxic free radicals. By taking advantage of this unique chemical agent, we have established melanoma-targeting intracellular hyperthermia by conjugating NPrCAP with magnetite nanoparticles (NPrCAP/M) upon exposure to an alternating magnetic field (AMF). This treatment causes cytotoxic reaction as well as heat shock responses, leading to elicitation of antitumor immune response, which was proved by tumor rechallenge test and CTL induction. We found the level of heat shock protein 72 (Hsp72) to be increased in the cell lysate and culture supernatant after intracellular hyperthermia. Melanoma-specific CD8+ T-cell response to dendritic cells loaded with hyperthermia-treated tumor lysate was enhanced when compared with non-treated tumor lysate. When heat shock protein, particularly Hsp72, was immuno-depleted from hyperthermia-treated tumor cell lysate, specific CD8+ T-cell response was abolished. Thus, it is suggested that antitumor immune response induced by hyperthermia using NPrCAP/M is derived from the release of HSP-peptide complex from degraded tumor cells. Therefore, this chemo-thermo-immuno (CTI)-therapy might be effective not only for primary melanoma but also for distant metastasis because of induction of systemic antimelanoma immune responses.

UR - http://www.scopus.com/inward/record.url?scp=77955934191&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=77955934191&partnerID=8YFLogxK

U2 - 10.1111/j.1349-7006.2010.01623.x

DO - 10.1111/j.1349-7006.2010.01623.x

M3 - Article

C2 - 20594194

AN - SCOPUS:77955934191

VL - 101

SP - 1939

EP - 1946

JO - Cancer Science

JF - Cancer Science

SN - 1347-9032

IS - 9

ER -