Membrane currents evoked by histamine in rabbit basilar artery

Masahiro Kamouchi, Rika Ogata, Masatoshi Fujishima, Yushi Ito, Kenji Kitamura

研究成果: ジャーナルへの寄稿記事

12 引用 (Scopus)

抄録

The membrane current evoked by histamine in isolated smooth muscle cells from rabbit basilar artery was investigated using the perforated-patch technique. When 10 μM histamine was applied in the bath at a holding potential of 60 mV, an inward current (79.2 ± 55.8 pA) was transiently activated. An outward current was additionally evoked by 10 μM histamine when the membrane was held at -40 mV or less negative potentials. The outward but not the inward current was completely blocked by 100 nM charybdotoxin. A higher concentration of histamine (30 μM) failed to produce the inward current (3.4 ± 4.8 pA) when Cl- concentration in the pipette was reduced. The apparent reversal potential of the inward current induced by histamine in physiological salt solution, in high-tetraethylammonium (TEA+) solution (bath), or in low-Cl- solution (pipette) was -6.3 ± 4.4, -7.5 ± 4.9, or - 45.8 ± 8.5 mV, respectively. Niflumic acid (100 μM) reversibly blocked the inward current, which was also blocked by 10 μM pyrilamine but not by 10 μM cimetidine. When histamine was continuously applied in the bath, spontaneous transient inward currents were generated. Removal of external Ca2+ or addition of 1 μM nicardipine or 2 mM caffeine reduced the amplitude of the histamine-induced inward current. These results suggest that histamine induces an inward current via H1 receptors at the resting membrane potential, possibly due to activation of Cl- currents. The Cl- inward current might be generated by elevation of intracellular Ca2+ via histamine receptors. The inward current may also contribute to control of the Ca2+ influx via a change in the membrane potential.

元の言語英語
ページ(範囲)H638-H647
ジャーナルAmerican Journal of Physiology - Heart and Circulatory Physiology
272
発行部数2 41-2
出版物ステータス出版済み - 2 1 1997

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Basilar Artery
Histamine
Rabbits
Membranes
Baths
Membrane Potentials
Niflumic Acid
Charybdotoxin
Pyrilamine
Nicardipine
Histamine Receptors
Histamine H1 Receptors
Tetraethylammonium
Cimetidine
Caffeine
Smooth Muscle Myocytes
Salts

All Science Journal Classification (ASJC) codes

  • Physiology
  • Cardiology and Cardiovascular Medicine
  • Physiology (medical)

これを引用

Membrane currents evoked by histamine in rabbit basilar artery. / Kamouchi, Masahiro; Ogata, Rika; Fujishima, Masatoshi; Ito, Yushi; Kitamura, Kenji.

:: American Journal of Physiology - Heart and Circulatory Physiology, 巻 272, 番号 2 41-2, 01.02.1997, p. H638-H647.

研究成果: ジャーナルへの寄稿記事

Kamouchi, M, Ogata, R, Fujishima, M, Ito, Y & Kitamura, K 1997, 'Membrane currents evoked by histamine in rabbit basilar artery', American Journal of Physiology - Heart and Circulatory Physiology, 巻. 272, 番号 2 41-2, pp. H638-H647.
Kamouchi, Masahiro ; Ogata, Rika ; Fujishima, Masatoshi ; Ito, Yushi ; Kitamura, Kenji. / Membrane currents evoked by histamine in rabbit basilar artery. :: American Journal of Physiology - Heart and Circulatory Physiology. 1997 ; 巻 272, 番号 2 41-2. pp. H638-H647.
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