Meta-fermentation system with a mixed culture for the production of optically pure l-lactic acid can be reconstructed using the minimum isolates with a simplified pH control strategy

Siyuan Yue, Takaharu Mizoguchi, Toshiya Kohara, Min Zhang, Kota Watanabe, Hirokuni Miyamoto, Yukihiro Tashiro, Kenji Sakai

研究成果: Contribution to journalArticle査読

抄録

Meta-l-lactic acid fermentation from non-treated kitchen refuse was reconstructed using a combination of isolated bacterial strains under several pH control strategies. The meta-fermentation system was successfully reconstructed using a combination of Weizmannia coagulans MN-07, Caldibacillus thermoamylovorans OM55-6, and Caldibacillus hisashii N-11 strains. Additionally, a simplified constant pH control strategy was employed, which decreased fermentation time and increased production. The optimum pH (6.5) for the reconstructed meta-fermentation was favorable for the respective pure cultures of the three selected strains. The l-lactic acid production performance of the reconstructed meta-fermentation system was as follows: concentration, 24.5 g L−1; optical purity, 100%; productivity, 0.341 g L−1 h−1; yield, 1.06 g g−1. These results indicated that constant pH control was effective in the reconstructed meta-fermentation with the best performance of l-lactic acid production at pH optimal for the selected bacterial growth, while the switching from swing pH control would suppress the activities of unfavorable bacterial species in un-isolated meta-fermentation.

本文言語英語
論文番号2100277
ジャーナルBiotechnology Journal
16
11
DOI
出版ステータス出版済み - 11 2021

All Science Journal Classification (ASJC) codes

  • 応用微生物学とバイオテクノロジー
  • 分子医療

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