Metabolic engineering for isopropanol production by an engineered cyanobacterium, Synechococcus elongatus PCC 7942, under photosynthetic conditions

Yasutaka Hirokawa, Yudai Dempo, Eiichiro Fukusaki, Taizo Hanai

研究成果: ジャーナルへの寄稿記事

12 引用 (Scopus)

抄録

Cyanobacteria engineered for production of biofuels and biochemicals from carbon dioxide represent a promising area of research in relation to a sustainable economy. Previously, we have succeeded in producing isopropanol from cellular acetyl-CoA by means of Synechococcus elongatus PCC 7942 into which a synthetic metabolic pathway was introduced. The isopropanol production by this synthetic metabolic pathway requires acetate; therefore, the cells grown under photosynthetic conditions have to be transferred to a dark and anaerobic conditions to produce acetate. In this study, we achieved acetate production under photosynthetic conditions by S. elongatus PCC 7942 into which we introduced the pta gene encoding phosphate acetyltransferase from Escherichia coli. The metabolic modification (via pta introduction) of the isopropanol-producing strain enabled production of isopropanol under photosynthetic conditions. During 14 days of production, the titer of isopropanol reached 0.55 mM (33.1 mg/l) with an intermediate product, acetone, at 0.21 mM (12.2 mg/l).

元の言語英語
ページ(範囲)39-45
ページ数7
ジャーナルJournal of Bioscience and Bioengineering
123
発行部数1
DOI
出版物ステータス出版済み - 1 1 2017

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Metabolic engineering
Synechococcus
Metabolic Engineering
2-Propanol
Cyanobacteria
Acetates
Metabolic Networks and Pathways
Phosphate Acetyltransferase
Acetyl Coenzyme A
Gene encoding
Biofuels
Acetone
Carbon Dioxide
Escherichia coli
Carbon dioxide
Phosphates
Cells
Research
Genes

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

これを引用

Metabolic engineering for isopropanol production by an engineered cyanobacterium, Synechococcus elongatus PCC 7942, under photosynthetic conditions. / Hirokawa, Yasutaka; Dempo, Yudai; Fukusaki, Eiichiro; Hanai, Taizo.

:: Journal of Bioscience and Bioengineering, 巻 123, 番号 1, 01.01.2017, p. 39-45.

研究成果: ジャーナルへの寄稿記事

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AU - Hanai, Taizo

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