Metabolism of mono- and dichloro-dibenzo-p-dioxins by Phanerochaete chrysosporium cytochromes P450

Noriyuki Kasai, Shin Ichi Ikushiro, Raku Shinkyo, Kaori Yasuda, Shinji Hirosue, Akira Arisawa, Hirofumi Ichinose, Hiroyuki Wariishi, Toshiyuki Sakaki

研究成果: ジャーナルへの寄稿記事

31 引用 (Scopus)

抄録

The white-rot fungus Phanerochaete chrysosporium possesses biodegradative capabilities of polychlorinated dibenzo-p-dioxins (PCDDs). One hundred twenty yeast clones expressing individual P450s of P. chrysosporum (PcCYPs), generated in our previous efforts, were screened for transformation of dioxin, and 40 positive clones were obtained. Of these clones, six clones showed metabolism of 2-chloro-dibenzo-p-dioxin, and a microsomal PcCYP designated as PcCYP11a3 showed much higher activity than any other PcCYPs. The turnover numbers of hydroxylation activities of PcCYP11a3 toward 1-MCDD (58 min-1) and 2-MCDD (13 min-1) are more than 200 times higher than those of previously reported PcCYP65a2. In addition, PcCYP11a3 catalyzes hydroxylation of 2,3-dichloro-dibenzo-p-dioxin. To our best knowledge, PcCYP11a3 has the highest activity toward PCDDs among the known CYPs derived from microorganisms. Although PcCYP11a3 showed no detectable activity toward 2,7-dichloro-dibenzo-p- dioxin and 2,3,7-trichloro-dibenzo-p-dioxin, PcCYP11a3 is promising as a template whose activity would be enhanced by site-directed mutagenesis.

元の言語英語
ページ(範囲)773-780
ページ数8
ジャーナルApplied Microbiology and Biotechnology
86
発行部数2
DOI
出版物ステータス出版済み - 3 1 2010

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Phanerochaete
Cytochrome P-450 Enzyme System
Clone Cells
Hydroxylation
Dioxins
Site-Directed Mutagenesis
Fungi
Yeasts
dibenzo(1,4)dioxin
Polychlorinated Dibenzodioxins

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Applied Microbiology and Biotechnology

これを引用

Kasai, N., Ikushiro, S. I., Shinkyo, R., Yasuda, K., Hirosue, S., Arisawa, A., ... Sakaki, T. (2010). Metabolism of mono- and dichloro-dibenzo-p-dioxins by Phanerochaete chrysosporium cytochromes P450. Applied Microbiology and Biotechnology, 86(2), 773-780. https://doi.org/10.1007/s00253-009-2413-x

Metabolism of mono- and dichloro-dibenzo-p-dioxins by Phanerochaete chrysosporium cytochromes P450. / Kasai, Noriyuki; Ikushiro, Shin Ichi; Shinkyo, Raku; Yasuda, Kaori; Hirosue, Shinji; Arisawa, Akira; Ichinose, Hirofumi; Wariishi, Hiroyuki; Sakaki, Toshiyuki.

:: Applied Microbiology and Biotechnology, 巻 86, 番号 2, 01.03.2010, p. 773-780.

研究成果: ジャーナルへの寄稿記事

Kasai, Noriyuki ; Ikushiro, Shin Ichi ; Shinkyo, Raku ; Yasuda, Kaori ; Hirosue, Shinji ; Arisawa, Akira ; Ichinose, Hirofumi ; Wariishi, Hiroyuki ; Sakaki, Toshiyuki. / Metabolism of mono- and dichloro-dibenzo-p-dioxins by Phanerochaete chrysosporium cytochromes P450. :: Applied Microbiology and Biotechnology. 2010 ; 巻 86, 番号 2. pp. 773-780.
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abstract = "The white-rot fungus Phanerochaete chrysosporium possesses biodegradative capabilities of polychlorinated dibenzo-p-dioxins (PCDDs). One hundred twenty yeast clones expressing individual P450s of P. chrysosporum (PcCYPs), generated in our previous efforts, were screened for transformation of dioxin, and 40 positive clones were obtained. Of these clones, six clones showed metabolism of 2-chloro-dibenzo-p-dioxin, and a microsomal PcCYP designated as PcCYP11a3 showed much higher activity than any other PcCYPs. The turnover numbers of hydroxylation activities of PcCYP11a3 toward 1-MCDD (58 min-1) and 2-MCDD (13 min-1) are more than 200 times higher than those of previously reported PcCYP65a2. In addition, PcCYP11a3 catalyzes hydroxylation of 2,3-dichloro-dibenzo-p-dioxin. To our best knowledge, PcCYP11a3 has the highest activity toward PCDDs among the known CYPs derived from microorganisms. Although PcCYP11a3 showed no detectable activity toward 2,7-dichloro-dibenzo-p- dioxin and 2,3,7-trichloro-dibenzo-p-dioxin, PcCYP11a3 is promising as a template whose activity would be enhanced by site-directed mutagenesis.",
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