Metabolite analysis by supercritical fluid chromatography

Atsuki Matsubara, Eiichiro Fukusaki, Takeshi Bamba

研究成果: Contribution to journalReview article査読

30 被引用数 (Scopus)

抄録

Owing to its favorable properties, such as low viscosity and high diffusivity, a supercritical fluid can be used as the mobile phase in chromatography. Supercritical fluid chromatography (SFC) can provide high-speed and high-resolution separation. Since supercritical carbon dioxide (SCCO 2), which is generally used as the mobile phase in SFC, is automatically emitted at room temperature, SFC is most commonly used as a preparative method. However, SFC can also be used to perform high-precision biomolecular analysis, especially for hydrophobic metabolites, because of the low polarity of SCCO2. The use of a mass spectrometer with SFC can widen the scope of application of SFC to bioanalysis. In this review, we summarize practical application of SFC as a tool for the analysis of metabolites in real biological samples.

本文言語英語
ページ(範囲)27-34
ページ数8
ジャーナルBioanalysis
2
1
DOI
出版ステータス出版済み - 1 1 2010
外部発表はい

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Pharmacology, Toxicology and Pharmaceutics(all)
  • Clinical Biochemistry
  • Medical Laboratory Technology

フィンガープリント 「Metabolite analysis by supercritical fluid chromatography」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル