Method for rapid distinction of Bombyx mandarina (Japan) from B. mandarina (China) based on rDNA sequence differences

Yuichi Kawanishi, Yutaka Banno, Hirofumi Fujimoto, Si Kab Nho, Zhenli Tu, Kazuei Mita, Kozo Tsuchida, Naoko Takada, Hideaki Maekawa, Yumiko Nakajima

研究成果: ジャーナルへの寄稿記事

抄録

The EcoRI site is located between the 5.8S and 28S rRNA genes in Bombyx mandarina (Japan) (Japanese type rDNA) but is absent in the rDNA of B. mandarina (China)(B. mori type rDNA) including the domesticated B. mori. Digestion of PCR products amplified by primers of both flanking sides of this EcoRI site with EcoRI should only be successful for amplification products of B. mandarina (Japan) containing Japanese type rDNA. The distribution of Japanese type rDNA in Japan, Korea, and China was determined using this rapid detection method. Japanese type was not detected in China or in Korea despite having 27 chromosomes. These findings suggest that the choromosome fusion and the rDNA type separation were independent evolutionary events. Invasion of B. mori chromosome #11 into Japanese B. mandarina (Japan) was examined.

元の言語英語
ページ(範囲)79-85
ページ数7
ジャーナルJournal of Insect Biotechnology and Sericology
77
発行部数2
出版物ステータス出版済み - 6 1 2008

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Bombyx
Chromosomes
Ribosomal DNA
rapid methods
China
Japan
Morus
Korean Peninsula
Amplification
Fusion reactions
Genes
Korea
chromosomes
Bombyx mori
Chromosomes, Human, Pair 11
digestion
ribosomal RNA
rRNA Genes
Bombyx mandarina
Digestion

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Applied Microbiology and Biotechnology
  • Business, Management and Accounting(all)
  • Agricultural and Biological Sciences(all)
  • Insect Science
  • Industrial and Manufacturing Engineering

これを引用

Method for rapid distinction of Bombyx mandarina (Japan) from B. mandarina (China) based on rDNA sequence differences. / Kawanishi, Yuichi; Banno, Yutaka; Fujimoto, Hirofumi; Nho, Si Kab; Tu, Zhenli; Mita, Kazuei; Tsuchida, Kozo; Takada, Naoko; Maekawa, Hideaki; Nakajima, Yumiko.

:: Journal of Insect Biotechnology and Sericology, 巻 77, 番号 2, 01.06.2008, p. 79-85.

研究成果: ジャーナルへの寄稿記事

Kawanishi, Y, Banno, Y, Fujimoto, H, Nho, SK, Tu, Z, Mita, K, Tsuchida, K, Takada, N, Maekawa, H & Nakajima, Y 2008, 'Method for rapid distinction of Bombyx mandarina (Japan) from B. mandarina (China) based on rDNA sequence differences', Journal of Insect Biotechnology and Sericology, 巻. 77, 番号 2, pp. 79-85.
Kawanishi, Yuichi ; Banno, Yutaka ; Fujimoto, Hirofumi ; Nho, Si Kab ; Tu, Zhenli ; Mita, Kazuei ; Tsuchida, Kozo ; Takada, Naoko ; Maekawa, Hideaki ; Nakajima, Yumiko. / Method for rapid distinction of Bombyx mandarina (Japan) from B. mandarina (China) based on rDNA sequence differences. :: Journal of Insect Biotechnology and Sericology. 2008 ; 巻 77, 番号 2. pp. 79-85.
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abstract = "The EcoRI site is located between the 5.8S and 28S rRNA genes in Bombyx mandarina (Japan) (Japanese type rDNA) but is absent in the rDNA of B. mandarina (China)(B. mori type rDNA) including the domesticated B. mori. Digestion of PCR products amplified by primers of both flanking sides of this EcoRI site with EcoRI should only be successful for amplification products of B. mandarina (Japan) containing Japanese type rDNA. The distribution of Japanese type rDNA in Japan, Korea, and China was determined using this rapid detection method. Japanese type was not detected in China or in Korea despite having 27 chromosomes. These findings suggest that the choromosome fusion and the rDNA type separation were independent evolutionary events. Invasion of B. mori chromosome #11 into Japanese B. mandarina (Japan) was examined.",
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AU - Nho, Si Kab

AU - Tu, Zhenli

AU - Mita, Kazuei

AU - Tsuchida, Kozo

AU - Takada, Naoko

AU - Maekawa, Hideaki

AU - Nakajima, Yumiko

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