Method for Structural Determination of Lipid-Derived Radicals

研究成果: Contribution to journalArticle査読

2 被引用数 (Scopus)

抄録

Diversified oxidized-lipid molecules are responsible for inflammation and cell death, including ferroptosis. Lipid radicals are the source of these oxidized lipids, which are the initial key molecules in the lipid peroxidation chain reaction. However, owing to their extremely high reactivity and short half-life, an established detection technique is not available. Here, we propose a high-performance liquid chromatography fluorometry and high-resolution tandem mass spectrometry system combined with a fluorescent probe as a structural analysis method for lipid-derived radicals. We detected 132 lipid-derived radicals, including 111 new species, from five polyunsaturated fatty acids. In addition, a database was constructed for which the initial fatty acid could be determined using the radical structure. Further, 12 endogenous lipid-derived radicals were identified in carcinogen-induced liver cancer mouse models. Therefore, this method and its corresponding database will provide novel insights into mechanisms underlying the lipid peroxidation, including the associated inflammation and ferroptosis.

本文言語英語
ページ(範囲)6993-7002
ページ数10
ジャーナルAnalytical Chemistry
92
10
DOI
出版ステータス出版済み - 5 19 2020

All Science Journal Classification (ASJC) codes

  • 分析化学

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