Methods for protein-protein interaction analysis

Keiji Kito, Takashi Ito

研究成果: 著書/レポートタイプへの貢献

抄録

Protein-protein interactions (PPIs) play key roles in various aspects of cellular regulation. Accordingly, PPI analysis is crucial for systems-level understanding of any biological process. This chapter describes two major methods for PPI analysis, namely, mass spectrometry (MS)-based approaches and yeast two-hybrid (Y2H)-based ones. Both methods have been the major driving forces of interactome mapping to reveal the characteristics of global PPI networks. Furthermore, MS-based methods enable a quantitative analysis of interaction, thereby providing insight into system dynamics. Alternatively, Y2H-based methods provide interaction- defective and separation-of-function alleles, which are useful for system perturbation. These two methods would thus contribute not only to system identification but also to system analysis, thereby serving as invaluable tools for systems biologists.

元の言語英語
ホスト出版物のタイトルIntroduction to Systems Biology
出版者Humana Press
ページ160-182
ページ数23
ISBN(印刷物)9781588297068
DOI
出版物ステータス出版済み - 12 1 2007

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Proteins
Mass Spectrometry
Mass spectrometry
Protein Interaction Maps
Biological Phenomena
Systems Analysis
Yeast
Yeasts
Alleles
Identification (control systems)
Dynamical systems
Systems analysis
Chemical analysis

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)

これを引用

Kito, K., & Ito, T. (2007). Methods for protein-protein interaction analysis. : Introduction to Systems Biology (pp. 160-182). Humana Press. https://doi.org/10.1007/978-1-59745-531-2_9

Methods for protein-protein interaction analysis. / Kito, Keiji; Ito, Takashi.

Introduction to Systems Biology. Humana Press, 2007. p. 160-182.

研究成果: 著書/レポートタイプへの貢献

Kito, K & Ito, T 2007, Methods for protein-protein interaction analysis. : Introduction to Systems Biology. Humana Press, pp. 160-182. https://doi.org/10.1007/978-1-59745-531-2_9
Kito K, Ito T. Methods for protein-protein interaction analysis. : Introduction to Systems Biology. Humana Press. 2007. p. 160-182 https://doi.org/10.1007/978-1-59745-531-2_9
Kito, Keiji ; Ito, Takashi. / Methods for protein-protein interaction analysis. Introduction to Systems Biology. Humana Press, 2007. pp. 160-182
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