Mice lacking connexin45 conditionally in cardiac myocytes display embryonic lethality similar to that of germline knockout mice without endocardial cushion defect

Kiyomasa Nishii, Madoka Kumai, Katsuko Egashira, Takeshi Miwa, Kanako Hashizume, Yumi Miyano, Yosaburo Shibata

研究成果: Contribution to journalArticle査読

47 被引用数 (Scopus)

抄録

The gap junction protein connexin45-deficient (Cx45-KO) mice die shortly after the hearts begin to beat. In addition to the heart defect, they also show defective vascular development which may be closely related with the cardiac phenotype. Therefore, we created mice whose floxed-Cx45 locus could be removed conditionally. We utilized cardiac α-actin-Cre transgenic mice to investigate the specific cardiac muscular function of Cx45 in vivo. The resultant conditional mutants were lethal, showing conduction block similar to that of the Cx45-KO mice. Unlike Cx45-KO, development of the endocardial cushion was not disrupted in the conditional mutants. X-gal staining was detected in the embryonic cardiac myocytes as a hallmark of Cre-loxP mediated floxed-Cx45 deletion. These results reconfirm the requirement of Cx45 for developing cardiac myocytes. These also indicate that establishing the first contractions is a crucial task for the early hearts.

本文言語英語
ページ(範囲)365-369
ページ数5
ジャーナルCell Communication and Adhesion
10
4-6
DOI
出版ステータス出版済み - 2003
外部発表はい

All Science Journal Classification (ASJC) codes

  • 臨床生化学
  • 細胞生物学

フィンガープリント

「Mice lacking connexin45 conditionally in cardiac myocytes display embryonic lethality similar to that of germline knockout mice without endocardial cushion defect」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル