TY - JOUR
T1 - Micro-LED Array-Based Photo-Stimulation Devices for Optogenetics in Rat and Macaque Monkey Brains
AU - Ohta, Yasumi
AU - Guinto, Mark Christian
AU - Tokuda, Takashi
AU - Kawahara, Mamiko
AU - Haruta, Makito
AU - Takehara, Hironari
AU - Tashiro, Hiroyuki
AU - Sasagawa, Kiyotaka
AU - Onoe, Hirotaka
AU - Yamaguchi, Reona
AU - Koshimizu, Yoshinori
AU - Isa, Kaoru
AU - Isa, Tadashi
AU - Kobayashi, Kenta
AU - Akay, Yasemin M.
AU - Akay, Metin
AU - Ohta, Jun
N1 - Funding Information:
This work was supported in part by Core Research for Evolutional Science and Technology (CREST) of the Japan Science and Technology Agency (JST) under Grant JPMJCR1651, and in part by Grant-in-Aid for Scientific Research (A) (KAKENHI) of The Japan Society for the Promotion of Science (JSPS) under Grant JP18H03780.
Publisher Copyright:
© 2013 IEEE.
PY - 2021
Y1 - 2021
N2 - Optogenetics is a powerful tool for controlling biological functions using light. Optical fibers have been extensively utilized in optical stimulation devices for optogenetics. However, the use of optical fibers results in a small photo-stimulation region. In this study, micro-LED array devices were developed to achieve large-area photo-stimulation in the brain of a large animal, such as macaque monkeys. Planar and linear micro-LED array devices were designed and fabricated to photo-stimulate the prefrontal cortex (PFC) and ventral tegmental area (VTA) of the brain and induce a neurochemical response. Device operation, optical intensity, and safety were first characterized using rats. Subsequently, the devices were used to photo-stimulate the brain of macaque monkeys. In addition, microdialysis in the PFC was performed. The devices detected modulated levels of dopamine in the brains. Thus, the photo-stimulation of both the PFC and VTA were successfully achieved, and the effectiveness of the developed micro-LED array devices was demonstrated. The study will help facilitate further studies on micro-LED array stimulation for system-wide optogenetic manipulation in large animals.
AB - Optogenetics is a powerful tool for controlling biological functions using light. Optical fibers have been extensively utilized in optical stimulation devices for optogenetics. However, the use of optical fibers results in a small photo-stimulation region. In this study, micro-LED array devices were developed to achieve large-area photo-stimulation in the brain of a large animal, such as macaque monkeys. Planar and linear micro-LED array devices were designed and fabricated to photo-stimulate the prefrontal cortex (PFC) and ventral tegmental area (VTA) of the brain and induce a neurochemical response. Device operation, optical intensity, and safety were first characterized using rats. Subsequently, the devices were used to photo-stimulate the brain of macaque monkeys. In addition, microdialysis in the PFC was performed. The devices detected modulated levels of dopamine in the brains. Thus, the photo-stimulation of both the PFC and VTA were successfully achieved, and the effectiveness of the developed micro-LED array devices was demonstrated. The study will help facilitate further studies on micro-LED array stimulation for system-wide optogenetic manipulation in large animals.
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U2 - 10.1109/ACCESS.2021.3111666
DO - 10.1109/ACCESS.2021.3111666
M3 - Article
AN - SCOPUS:85114735274
SN - 2169-3536
VL - 9
SP - 127937
EP - 127949
JO - IEEE Access
JF - IEEE Access
ER -