Microanalysis of Nucleic Acids Using the Limulus G Test

Takahisa Anada, Kazuya Koumoto, Masami Mizu, Ryouji Karinaga, Kazuo Sakurai, Seiji Shinkai

研究成果: Contribution to journalArticle査読

1 被引用数 (Scopus)

抄録

The limulus G test has been used as a quantitative analysis of (1→3)-β-D-glucans, including schizophyllan (SPG) and curdlan. The present work extended the limulus G test to detect polynucleotide/SPG complexes. The complex showed an extremely sensitive response to the test, compared with SPG itself. The minimum concentration of the complex to show the response is almost 10-times as small as that of SPG itself, indicating the possibility to detect (1→3)-β-D-glucans or/and polynucleotides on the pico gram/ml scale.

本文言語英語
ページ(範囲)1567-1568
ページ数2
ジャーナルanalytical sciences
19
12
DOI
出版ステータス出版済み - 12 2003
外部発表はい

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry

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