Microarray technologies for intracellular kinome analysis

研究成果: ジャーナルへの寄稿記事

5 引用 (Scopus)

抄録

Microarray-based kinomics, which measure the enzymatic activity or the presence of intracellular protein kinases, are now regarded as alternative tools to conventional mass spectrometry-based kinomics for examining intracellular kinomics. Here, we reviewed the principal advantages, recent progress, and remaining problems of representative microarray- based kinomics, including substrate peptide and protein microarrays, anti-protein kinase antibody microarrays, and reverse protein microarrays. Microarray-based kinomics are not as good at quantitative evaluation of kinomics as the conventional mass spectrometry-based kinomics. However, their simplicity and high throughput make the microarraybased kinomics unique tools, being especially suited for a practical analysis; monitoring drug effects on cellular kinomics as a tool for drug development, and for the diagnosis and prognosis of diseases based on kinomics.

元の言語英語
ページ(範囲)2542-2552
ページ数11
ジャーナルCurrent Medicinal Chemistry
21
発行部数22
DOI
出版物ステータス出版済み - 1 1 2014

Fingerprint

Protein Array Analysis
Microarrays
Protein Kinases
Mass Spectrometry
Technology
Drug Monitoring
Peptides
Mass spectrometry
Antibodies
Pharmaceutical Preparations
Proteins
Throughput
Monitoring
Substrates

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Medicine
  • Pharmacology
  • Drug Discovery
  • Organic Chemistry

これを引用

Microarray technologies for intracellular kinome analysis. / Yamamoto, T.; Mori, Takeshi; Katayama, Yoshiki.

:: Current Medicinal Chemistry, 巻 21, 番号 22, 01.01.2014, p. 2542-2552.

研究成果: ジャーナルへの寄稿記事

@article{ce7f3866901f4de3a63669c177597d07,
title = "Microarray technologies for intracellular kinome analysis",
abstract = "Microarray-based kinomics, which measure the enzymatic activity or the presence of intracellular protein kinases, are now regarded as alternative tools to conventional mass spectrometry-based kinomics for examining intracellular kinomics. Here, we reviewed the principal advantages, recent progress, and remaining problems of representative microarray- based kinomics, including substrate peptide and protein microarrays, anti-protein kinase antibody microarrays, and reverse protein microarrays. Microarray-based kinomics are not as good at quantitative evaluation of kinomics as the conventional mass spectrometry-based kinomics. However, their simplicity and high throughput make the microarraybased kinomics unique tools, being especially suited for a practical analysis; monitoring drug effects on cellular kinomics as a tool for drug development, and for the diagnosis and prognosis of diseases based on kinomics.",
author = "T. Yamamoto and Takeshi Mori and Yoshiki Katayama",
year = "2014",
month = "1",
day = "1",
doi = "10.2174/0929867321666131212154153",
language = "English",
volume = "21",
pages = "2542--2552",
journal = "Current Medicinal Chemistry",
issn = "0929-8673",
publisher = "Bentham Science Publishers B.V.",
number = "22",

}

TY - JOUR

T1 - Microarray technologies for intracellular kinome analysis

AU - Yamamoto, T.

AU - Mori, Takeshi

AU - Katayama, Yoshiki

PY - 2014/1/1

Y1 - 2014/1/1

N2 - Microarray-based kinomics, which measure the enzymatic activity or the presence of intracellular protein kinases, are now regarded as alternative tools to conventional mass spectrometry-based kinomics for examining intracellular kinomics. Here, we reviewed the principal advantages, recent progress, and remaining problems of representative microarray- based kinomics, including substrate peptide and protein microarrays, anti-protein kinase antibody microarrays, and reverse protein microarrays. Microarray-based kinomics are not as good at quantitative evaluation of kinomics as the conventional mass spectrometry-based kinomics. However, their simplicity and high throughput make the microarraybased kinomics unique tools, being especially suited for a practical analysis; monitoring drug effects on cellular kinomics as a tool for drug development, and for the diagnosis and prognosis of diseases based on kinomics.

AB - Microarray-based kinomics, which measure the enzymatic activity or the presence of intracellular protein kinases, are now regarded as alternative tools to conventional mass spectrometry-based kinomics for examining intracellular kinomics. Here, we reviewed the principal advantages, recent progress, and remaining problems of representative microarray- based kinomics, including substrate peptide and protein microarrays, anti-protein kinase antibody microarrays, and reverse protein microarrays. Microarray-based kinomics are not as good at quantitative evaluation of kinomics as the conventional mass spectrometry-based kinomics. However, their simplicity and high throughput make the microarraybased kinomics unique tools, being especially suited for a practical analysis; monitoring drug effects on cellular kinomics as a tool for drug development, and for the diagnosis and prognosis of diseases based on kinomics.

UR - http://www.scopus.com/inward/record.url?scp=84901927577&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84901927577&partnerID=8YFLogxK

U2 - 10.2174/0929867321666131212154153

DO - 10.2174/0929867321666131212154153

M3 - Article

C2 - 24358971

AN - SCOPUS:84901927577

VL - 21

SP - 2542

EP - 2552

JO - Current Medicinal Chemistry

JF - Current Medicinal Chemistry

SN - 0929-8673

IS - 22

ER -