Microbe-specific C3b deposition in the horseshoe crab complement system in a C2/factor B-dependent or -independent manner

Keisuke Tagawa, Toyoki Yoshihara, Toshio Shibata, Kazuki Kitazaki, Yuichi Endo, Teizo Fujita, Takumi Koshiba, Shun-Ichiro Kawabata

研究成果: ジャーナルへの寄稿記事

13 引用 (Scopus)

抄録

Complement C3 plays an essential role in the opsonization of pathogens in the mammalian complement system, whereas the molecular mechanism underlying C3 activation in invertebrates remains unknown. To understand the molecular mechanism of C3b deposition on microbes, we characterized two types of C2/factor B homologs (designated TtC2/Bf-1 and TtC2/Bf-2) identified from the horseshoe crab Tachypleus tridentatus. Although the domain architectures of TtC2/Bf-1 and TtC2/Bf-2 were identical to those of mammalian homologs, they contained five-repeated and seven-repeated complement control protein domains at their N-terminal regions, respectively. TtC2/Bf-1 and TtC2/Bf-2 were synthesized and glycosylated in hemocytes and secreted to hemolymph plasma, which existed in a complex with C3 (TtC3), and their activation by microbes was absolutely Mg2+-dependent. Flow cytometric analysis revealed that TtC3b deposition was Mg2+-dependent on Gram-positive bacteria or fungi, but not on Gram-negative bacteria. Moreover, this analysis demonstrated that Ca2+-dependent lectins (C-reactive protein-1 and tachylectin-5A) were required for TtC3b deposition on Gram-positive bacteria, and that a Ca2+-independent lectin (Tachypleus plasma lectin-1) was definitely indispensable for TtC3b deposition on fungi. In contrast, a horseshoe crab lipopolysaccharide-sensitive protease factor C was necessary and sufficient to deposit TtC3b on Gram-negative bacteria. We conclude that plasma lectins and factor C play key roles in microbe-specific TtC3b deposition in a C2/factor B-dependent or -independent manner.

元の言語英語
記事番号e36783
ジャーナルPloS one
7
発行部数5
DOI
出版物ステータス出版済み - 5 7 2012

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Merostomata
Horseshoe Crabs
Complement Factor B
Lectins
lectins
complement
Gram-Positive Bacteria
Gram-Negative Bacteria
Bacteria
microorganisms
Fungi
Gram-positive bacteria
Gram-negative bacteria
Hemocytes
Tachypleus
Tachypleus tridentatus
Complement C3
Hemolymph
Invertebrates
calcium

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

これを引用

Microbe-specific C3b deposition in the horseshoe crab complement system in a C2/factor B-dependent or -independent manner. / Tagawa, Keisuke; Yoshihara, Toyoki; Shibata, Toshio; Kitazaki, Kazuki; Endo, Yuichi; Fujita, Teizo; Koshiba, Takumi; Kawabata, Shun-Ichiro.

:: PloS one, 巻 7, 番号 5, e36783, 07.05.2012.

研究成果: ジャーナルへの寄稿記事

Tagawa, Keisuke ; Yoshihara, Toyoki ; Shibata, Toshio ; Kitazaki, Kazuki ; Endo, Yuichi ; Fujita, Teizo ; Koshiba, Takumi ; Kawabata, Shun-Ichiro. / Microbe-specific C3b deposition in the horseshoe crab complement system in a C2/factor B-dependent or -independent manner. :: PloS one. 2012 ; 巻 7, 番号 5.
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abstract = "Complement C3 plays an essential role in the opsonization of pathogens in the mammalian complement system, whereas the molecular mechanism underlying C3 activation in invertebrates remains unknown. To understand the molecular mechanism of C3b deposition on microbes, we characterized two types of C2/factor B homologs (designated TtC2/Bf-1 and TtC2/Bf-2) identified from the horseshoe crab Tachypleus tridentatus. Although the domain architectures of TtC2/Bf-1 and TtC2/Bf-2 were identical to those of mammalian homologs, they contained five-repeated and seven-repeated complement control protein domains at their N-terminal regions, respectively. TtC2/Bf-1 and TtC2/Bf-2 were synthesized and glycosylated in hemocytes and secreted to hemolymph plasma, which existed in a complex with C3 (TtC3), and their activation by microbes was absolutely Mg2+-dependent. Flow cytometric analysis revealed that TtC3b deposition was Mg2+-dependent on Gram-positive bacteria or fungi, but not on Gram-negative bacteria. Moreover, this analysis demonstrated that Ca2+-dependent lectins (C-reactive protein-1 and tachylectin-5A) were required for TtC3b deposition on Gram-positive bacteria, and that a Ca2+-independent lectin (Tachypleus plasma lectin-1) was definitely indispensable for TtC3b deposition on fungi. In contrast, a horseshoe crab lipopolysaccharide-sensitive protease factor C was necessary and sufficient to deposit TtC3b on Gram-negative bacteria. We conclude that plasma lectins and factor C play key roles in microbe-specific TtC3b deposition in a C2/factor B-dependent or -independent manner.",
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AU - Tagawa, Keisuke

AU - Yoshihara, Toyoki

AU - Shibata, Toshio

AU - Kitazaki, Kazuki

AU - Endo, Yuichi

AU - Fujita, Teizo

AU - Koshiba, Takumi

AU - Kawabata, Shun-Ichiro

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