Microchip analysis of plant glucosinolates

Maged Fouad, Mohammad Jabasini, Noritada Kaji, Kazuyoshi Terasaka, Manabu Tokeshi, Hajime Mizukami, Yoshinobu Baba

研究成果: ジャーナルへの寄稿記事

12 引用 (Scopus)

抄録

We describe a new and selective analytical method for the separation and quantitation of plant glucosinolates. The new method, which utilizes microchip CE (μ-CE) with fluorescence detection, circumvents the multistep procedures characteristic of conventional methods. Glucosinolates form charge transfer complexes with the xanthene dyes phloxine-B and eosin-B. The glucosinolates-phloxine-B complex cannot be excited at 470 nm. Thus, the decrease in peak intensity of phloxine-B after complex formation is used to quantitatively measure total glucosinolates in Arabidopsis thaliana seeds. For qualitative analysis, complex formation with eosin-B is used. The sensitivity of eosin-B detection at excitation/emission 470 nm/540 nm was low. However, sensitivity increased following complex formation with sinigrin (≥3 μg/mL). A batch-learning, self-organizing map was applied to visualize and organize analytical data into 2-D matrix with similar and related data clustered together or near each other. This organized matrix was used to optimize electrophoretic conditions for the analysis. This study suggests potential applications of μ-CE in plant metabolomics analyses without use of labeling fluorophores.

元の言語英語
ページ(範囲)2280-2287
ページ数8
ジャーナルELECTROPHORESIS
29
発行部数11
DOI
出版物ステータス出版済み - 6 1 2008
外部発表Yes

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Microchip Analytical Procedures
Eosine I Bluish
Glucosinolates
Xanthenes
Metabolomics
Fluorophores
Self organizing maps
Arabidopsis
Labeling
Seed
Charge transfer
Seeds
Coloring Agents
Fluorescence
Learning

All Science Journal Classification (ASJC) codes

  • Clinical Biochemistry

これを引用

Fouad, M., Jabasini, M., Kaji, N., Terasaka, K., Tokeshi, M., Mizukami, H., & Baba, Y. (2008). Microchip analysis of plant glucosinolates. ELECTROPHORESIS, 29(11), 2280-2287. https://doi.org/10.1002/elps.200700635

Microchip analysis of plant glucosinolates. / Fouad, Maged; Jabasini, Mohammad; Kaji, Noritada; Terasaka, Kazuyoshi; Tokeshi, Manabu; Mizukami, Hajime; Baba, Yoshinobu.

:: ELECTROPHORESIS, 巻 29, 番号 11, 01.06.2008, p. 2280-2287.

研究成果: ジャーナルへの寄稿記事

Fouad, M, Jabasini, M, Kaji, N, Terasaka, K, Tokeshi, M, Mizukami, H & Baba, Y 2008, 'Microchip analysis of plant glucosinolates', ELECTROPHORESIS, 巻. 29, 番号 11, pp. 2280-2287. https://doi.org/10.1002/elps.200700635
Fouad M, Jabasini M, Kaji N, Terasaka K, Tokeshi M, Mizukami H その他. Microchip analysis of plant glucosinolates. ELECTROPHORESIS. 2008 6 1;29(11):2280-2287. https://doi.org/10.1002/elps.200700635
Fouad, Maged ; Jabasini, Mohammad ; Kaji, Noritada ; Terasaka, Kazuyoshi ; Tokeshi, Manabu ; Mizukami, Hajime ; Baba, Yoshinobu. / Microchip analysis of plant glucosinolates. :: ELECTROPHORESIS. 2008 ; 巻 29, 番号 11. pp. 2280-2287.
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