Microchip electrophoresis for detection of circle-to-circle amplification products towards sensitive and rapid DNA analysis

Laili Mahmoudian, Jonas Melin, Mohamad Reza Mohamadi, Keiko Yamada, Michio Ohta, Noritada Kaji, Manabu Tokeshi, Mats Nilsson, Yoshinobu Baba

研究成果: Contribution to journalArticle査読

4 被引用数 (Scopus)

抄録

A new method for fast and precise analysis of circle-to-circle amplification (C2CA) products by microchip electrophoresis has been developed. Stable C2CA products were produced by applying a new enzymatic step to C2CA. Detection was carried out within 55s with RSD of migration time of 3.6% (n = 6) enabling reproducibility and high speed. A real sample of bacterial pathogen (V. Cholerae) at single nucleotide level was detected successfully based on this method.

本文言語英語
ページ(範囲)396-397
ページ数2
ジャーナルChemistry Letters
36
3
DOI
出版ステータス出版済み - 3 5 2007
外部発表はい

All Science Journal Classification (ASJC) codes

  • Chemistry(all)

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