Microelastic gradient gelatinous gels to induce cellular mechanotaxis

Satoru Kidoaki, Takehisa Matsuda

研究成果: ジャーナルへの寄稿記事

71 引用 (Scopus)

抄録

The understanding and realization of directional cell movement towards a harder region of a cell culture substrate surface, so-called mechanotaxis, might provide a solid basis for a functional artificial extracellular matrix, enabling manipulation and elucidation of cell motility. The photolithographic surface microelasticity patterning method was developed for fabricating a cell-adhesive hydrogel with a microelasticity gradient (MEG) surface using photocurable styrenated gelatin to investigate the condition of surface elasticity to induce mechanotaxis as a basis for such substrate-elasticity-dependent control of cell motility. Patterned MEG gels consisting of different absolute surface elasticities and elasticity jumps were prepared. Surface elasticity and its two-dimensional distribution were characterized by microindentation tests using atomic force microscopy (AFM). From analyses of trajectories of 3T3 cell movement on each prepared MEG gel, two critical criteria of the elasticity jump and the absolute elasticity to induce mechanotaxis were identified: (1) a high elasticity ratio between the hard region and the soft one, and (2) elasticity of the softer region to provide medium motility. Design of these conditions was found to be necessary for fabricating an artificial extracellular matrix to control or manipulate cell motility.

元の言語英語
ページ(範囲)225-230
ページ数6
ジャーナルJournal of Biotechnology
133
発行部数2
DOI
出版物ステータス出版済み - 1 20 2008

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Elasticity
Gels
Cell Movement
Extracellular Matrix
Cells
3T3 Cells
Atomic Force Microscopy
Hydrogel
Substrates
Gelatin
Cell culture
Hydrogels
Adhesives
Atomic force microscopy
Cell Culture Techniques
Trajectories

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

これを引用

Microelastic gradient gelatinous gels to induce cellular mechanotaxis. / Kidoaki, Satoru; Matsuda, Takehisa.

:: Journal of Biotechnology, 巻 133, 番号 2, 20.01.2008, p. 225-230.

研究成果: ジャーナルへの寄稿記事

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