Microfluidic sensor for the detection of DNA or protein by hybridization-based fluorescence enhancement or immunoassay-based fluorescence quenching

J. Wang, M. Aki, D. Onoshima, K. Arinaga, N. Kaji, M. Tokeshi, S. Fujita, N. Yokoyama, Y. Baba

研究成果: 書籍/レポート タイプへの寄稿会議への寄与

抄録

We have developed a novel optical DNA/protein sensor which consists of single-stranded (ss) DNA-Cy3 probes on gold surface and one line-shape polydimethylsiloxane (PDMS) microfluidic channel. This novel ssDNA-Cy3 probes, which does not require hairpin-like stem-loop conformation, show similar advantages as traditional molecular beacon technique in this microfluidic sensor. This chip-based optical sensor showed strong fluorescence enhancement by the binding of cDNA or cDNA-biotin. After introduction of cDNA-biotin, streptavidin can be determined by fluorescence quenching. This sensor showed strong affinity and high sensitivity toward streptavidin, the minimum detectable concentration for streptavidin was 1 pM, equating to an absolute detection limit of 50 amol in this sensor.

本文言語英語
ホスト出版物のタイトル14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010
ページ1514-1516
ページ数3
出版ステータス出版済み - 2010
外部発表はい
イベント14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010 - Groningen, オランダ
継続期間: 10月 3 201010月 7 2010

出版物シリーズ

名前14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010
3

その他

その他14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010
国/地域オランダ
CityGroningen
Period10/3/1010/7/10

!!!All Science Journal Classification (ASJC) codes

  • 制御およびシステム工学

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