Microfluidic stretching of DNA with fluorescent gold nanoparticle for optical/electron microscopic imaging of a single DNA methylation

Daiki Takeshita, Daisuke Onoshima, Yukawa Hiroshi, Takao Yasui, Noritada Kaji, Yoshinobu Baba

研究成果: 著書/レポートタイプへの貢献会議での発言

抄録

We combined a microfluidic stretch of a single DNA molecule and a visualization technique with fluorescent gold nanoparticle labeling, which can be observed with fluorescence microscope and scanning electron microscope (SEM) for imaging of methylated site on a single DNA molecule. It will realize bimodal microscope imaging of methylated site and enhance the imaging resolution down to nanometer level toward epigenetic analysis on the basis of high-resolution mapping of methylation sites.

元の言語英語
ホスト出版物のタイトル18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014
出版者Chemical and Biological Microsystems Society
ページ2348-2350
ページ数3
ISBN(電子版)9780979806476
出版物ステータス出版済み - 1 1 2014
外部発表Yes
イベント18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014 - San Antonio, 米国
継続期間: 10 26 201410 30 2014

出版物シリーズ

名前18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014

その他

その他18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014
米国
San Antonio
期間10/26/1410/30/14

Fingerprint

Microfluidics
Stretching
DNA
Gold
Nanoparticles
Imaging techniques
Electrons
Microscopes
Molecules
Methylation
Labeling
Electron microscopes
Visualization
Fluorescence
Scanning
DNA Methylation

All Science Journal Classification (ASJC) codes

  • Control and Systems Engineering

これを引用

Takeshita, D., Onoshima, D., Hiroshi, Y., Yasui, T., Kaji, N., & Baba, Y. (2014). Microfluidic stretching of DNA with fluorescent gold nanoparticle for optical/electron microscopic imaging of a single DNA methylation. : 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014 (pp. 2348-2350). (18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014). Chemical and Biological Microsystems Society.

Microfluidic stretching of DNA with fluorescent gold nanoparticle for optical/electron microscopic imaging of a single DNA methylation. / Takeshita, Daiki; Onoshima, Daisuke; Hiroshi, Yukawa; Yasui, Takao; Kaji, Noritada; Baba, Yoshinobu.

18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014. Chemical and Biological Microsystems Society, 2014. p. 2348-2350 (18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014).

研究成果: 著書/レポートタイプへの貢献会議での発言

Takeshita, D, Onoshima, D, Hiroshi, Y, Yasui, T, Kaji, N & Baba, Y 2014, Microfluidic stretching of DNA with fluorescent gold nanoparticle for optical/electron microscopic imaging of a single DNA methylation. : 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014. 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014, Chemical and Biological Microsystems Society, pp. 2348-2350, 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014, San Antonio, 米国, 10/26/14.
Takeshita D, Onoshima D, Hiroshi Y, Yasui T, Kaji N, Baba Y. Microfluidic stretching of DNA with fluorescent gold nanoparticle for optical/electron microscopic imaging of a single DNA methylation. : 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014. Chemical and Biological Microsystems Society. 2014. p. 2348-2350. (18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014).
Takeshita, Daiki ; Onoshima, Daisuke ; Hiroshi, Yukawa ; Yasui, Takao ; Kaji, Noritada ; Baba, Yoshinobu. / Microfluidic stretching of DNA with fluorescent gold nanoparticle for optical/electron microscopic imaging of a single DNA methylation. 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014. Chemical and Biological Microsystems Society, 2014. pp. 2348-2350 (18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014).
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