抄録
In vitro multiplication of Alocasia micholitziana 'Green Velvet', Alocasia X amazonica and Alocasia cuculata was achieved from axillary buds excised from the corms of greenhouse-grown plants. Shoots were developed directly within 6-8 weeks on the MS medium supplemented with 10 mgl -1 2-iP for A. micholitziana 'Green Velvet' or 10 mgl -1 BA for A. X amazonica and A. cuculata. Considering the quality of the shoots, high frequency of shoot regeneration was achieved on similar medium in subsequent subcultures for A. micholitziana 'Green Velvet' (5.0 shoots/culture). Five mgl -1 BA gave the best shoot regeneration in A. X amazonica and A. cuculata (4.6 and 5.0 shoots/culture, respectively). Shoots of the regenerated plants rooted abundantly on the hormone free MS within 4 weeks. Acclimatization was successful with approximately 100% of the in vitro plantlets which survived in a greenhouse. The regenerated plants seemed morphologically to be similar to the respective mother plants. The established procedure provided a basic technique to carry out the furture in vitro experiments in Alocasia.
| 元の言語 | 英語 |
|---|---|
| ページ（範囲） | 277-282 |
| ページ数 | 6 |
| ジャーナル | Journal of the Faculty of Agriculture, Kyushu University |
| 巻 | 47 |
| 発行部数 | 2 |
| 出版物ステータス | 出版済み - 2 1 2003 |
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All Science Journal Classification (ASJC) codes
- Biotechnology
- Agronomy and Crop Science
これを引用
Micropropagation of ornamental Alocasia. / Thao, Nguyen Thi Phuong; Miyajima, Ikuo; Ureshino, Kenji; Ozaki, Yukio; Okubo, Hiroshi.
：: Journal of the Faculty of Agriculture, Kyushu University, 巻 47, 番号 2, 01.02.2003, p. 277-282.研究成果: ジャーナルへの寄稿 › 記事
}
TY - JOUR
T1 - Micropropagation of ornamental Alocasia
AU - Thao, Nguyen Thi Phuong
AU - Miyajima, Ikuo
AU - Ureshino, Kenji
AU - Ozaki, Yukio
AU - Okubo, Hiroshi
PY - 2003/2/1
Y1 - 2003/2/1
N2 - In vitro multiplication of Alocasia micholitziana 'Green Velvet', Alocasia X amazonica and Alocasia cuculata was achieved from axillary buds excised from the corms of greenhouse-grown plants. Shoots were developed directly within 6-8 weeks on the MS medium supplemented with 10 mgl -1 2-iP for A. micholitziana 'Green Velvet' or 10 mgl -1 BA for A. X amazonica and A. cuculata. Considering the quality of the shoots, high frequency of shoot regeneration was achieved on similar medium in subsequent subcultures for A. micholitziana 'Green Velvet' (5.0 shoots/culture). Five mgl -1 BA gave the best shoot regeneration in A. X amazonica and A. cuculata (4.6 and 5.0 shoots/culture, respectively). Shoots of the regenerated plants rooted abundantly on the hormone free MS within 4 weeks. Acclimatization was successful with approximately 100% of the in vitro plantlets which survived in a greenhouse. The regenerated plants seemed morphologically to be similar to the respective mother plants. The established procedure provided a basic technique to carry out the furture in vitro experiments in Alocasia.
AB - In vitro multiplication of Alocasia micholitziana 'Green Velvet', Alocasia X amazonica and Alocasia cuculata was achieved from axillary buds excised from the corms of greenhouse-grown plants. Shoots were developed directly within 6-8 weeks on the MS medium supplemented with 10 mgl -1 2-iP for A. micholitziana 'Green Velvet' or 10 mgl -1 BA for A. X amazonica and A. cuculata. Considering the quality of the shoots, high frequency of shoot regeneration was achieved on similar medium in subsequent subcultures for A. micholitziana 'Green Velvet' (5.0 shoots/culture). Five mgl -1 BA gave the best shoot regeneration in A. X amazonica and A. cuculata (4.6 and 5.0 shoots/culture, respectively). Shoots of the regenerated plants rooted abundantly on the hormone free MS within 4 weeks. Acclimatization was successful with approximately 100% of the in vitro plantlets which survived in a greenhouse. The regenerated plants seemed morphologically to be similar to the respective mother plants. The established procedure provided a basic technique to carry out the furture in vitro experiments in Alocasia.
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UR - http://www.scopus.com/inward/citedby.url?scp=0041340622&partnerID=8YFLogxK
M3 - Article
AN - SCOPUS:0041340622
VL - 47
SP - 277
EP - 282
JO - Journal of the Faculty of Agriculture, Kyushu University
JF - Journal of the Faculty of Agriculture, Kyushu University
SN - 0023-6152
IS - 2
ER -