Minimal requirements for the nuclear localization of p27(Kip1), a cyclin-dependent kinase inhibitor

Ying Zeng, Katsuya Hirano, Mayumi Hirano, Junji Nishimura, Hideo Kanaide

研究成果: ジャーナルへの寄稿記事

50 引用 (Scopus)

抄録

p27(Kip1) is a cyclin-dependent kinase inhibitor, and its nuclear localization is a prerequisite for it to function as a cell cycle regulator. In the present study, the minimal requirement for the nuclear localization signal (NLS) of p27(Kip1) was determined by analyzing the localization of various mutants of p27(Kip1) tagged with green fluorescent protein (GFP) in HeLa cells and porcine aortic endothelial cells. Wild-type p27(Kip1) exclusively localized into nucleus, while GFP alone localized in both cytosol and nucleus. A comparison of various truncation mutants revealed residues 153-166 to be the minimal region necessary for nuclear localization. However, a fusion of this region to GFP showed cytoplasmic retention in addition to nuclear localization, thus suggesting that some extension flanking this region is required to achieve a full function of NLS. The site-directed mutation of the full-length p27(Kip1) therefore showed that four basic residues (K153, R154, K165, R166), especially R166, play a critical role in the nuclear localization of p27(Kip1). (C) 2000 Academic Press.

元の言語英語
ページ(範囲)37-42
ページ数6
ジャーナルBiochemical and Biophysical Research Communications
274
発行部数1
DOI
出版物ステータス出版済み - 7 21 2000

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Cyclin-Dependent Kinases
Green Fluorescent Proteins
Nuclear Localization Signals
Endothelial cells
HeLa Cells
Cytosol
Cell Cycle
Swine
Fusion reactions
Endothelial Cells
Cells
Mutation

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

これを引用

Minimal requirements for the nuclear localization of p27(Kip1), a cyclin-dependent kinase inhibitor. / Zeng, Ying; Hirano, Katsuya; Hirano, Mayumi; Nishimura, Junji; Kanaide, Hideo.

:: Biochemical and Biophysical Research Communications, 巻 274, 番号 1, 21.07.2000, p. 37-42.

研究成果: ジャーナルへの寄稿記事

Zeng, Ying ; Hirano, Katsuya ; Hirano, Mayumi ; Nishimura, Junji ; Kanaide, Hideo. / Minimal requirements for the nuclear localization of p27(Kip1), a cyclin-dependent kinase inhibitor. :: Biochemical and Biophysical Research Communications. 2000 ; 巻 274, 番号 1. pp. 37-42.
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AB - p27(Kip1) is a cyclin-dependent kinase inhibitor, and its nuclear localization is a prerequisite for it to function as a cell cycle regulator. In the present study, the minimal requirement for the nuclear localization signal (NLS) of p27(Kip1) was determined by analyzing the localization of various mutants of p27(Kip1) tagged with green fluorescent protein (GFP) in HeLa cells and porcine aortic endothelial cells. Wild-type p27(Kip1) exclusively localized into nucleus, while GFP alone localized in both cytosol and nucleus. A comparison of various truncation mutants revealed residues 153-166 to be the minimal region necessary for nuclear localization. However, a fusion of this region to GFP showed cytoplasmic retention in addition to nuclear localization, thus suggesting that some extension flanking this region is required to achieve a full function of NLS. The site-directed mutation of the full-length p27(Kip1) therefore showed that four basic residues (K153, R154, K165, R166), especially R166, play a critical role in the nuclear localization of p27(Kip1). (C) 2000 Academic Press.

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