miR-221 and miR-155 regulate human dendritic cell development, apoptosis, and IL-12 production through targeting of p27 kip1, KPC1, and SOCS-1

Changming Lu, Xin Huang, Xiaoxiao Zhang, Kristin Roensch, Qing Cao, Keiichi Nakayama, Bruce R. Blazar, Yan Zeng, Xianzheng Zhou

研究成果: ジャーナルへの寄稿記事

185 引用 (Scopus)

抄録

Dendritic cells (DCs) are potent antigen-presenting cells derived from hematopoietic progenitor cells and circulating monocytes. To investigate the role of microRNAs (miRNAs) during DC differentiation, maturation, and function, we profiled miRNA expression in human monocytes, immature DCs (imDCs), and mature DCs (mDCs). Stage-specific, differential expression of 27 miRNAs was found during monocyte differentiation into imDCs and mDCs. Among them, decreased miR-221 and increased miR-155 expression correlated with p27 kip1 accumulation in DCs. Silencing of miR-221 or overexpressing of miR-155 in DCs resulted in p27 kip1 protein increase and DC apoptosis. Moreover, mDCs from miR-155 -/- mice were less apoptotic than those from wild-type mice. Silencing of miR-155 expression had little effect on DC maturation but reduced IL-12p70 production, whereas miR-155 overexpression in mDCs enhanced IL-12p70 production. Kip1 ubiquitination-promoting complex 1, suppressor of cytokine signaling 1, and CD115 (M-CSFR) were functional targets of miR-155. Furthermore, we provide evidence that miR-155 indirectly regulated p27 kip1 protein level by targeting Kip1 ubiquitination-promoting complex 1. Thus, our study uncovered miRNA signatures during monocyte differentiation into DCs and the new regulatory role of miR-221 and miR-155 in DC apoptosis and IL-12p70 production.

元の言語英語
ページ(範囲)4293-4303
ページ数11
ジャーナルBlood
117
発行部数16
DOI
出版物ステータス出版済み - 4 21 2011

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Interleukin-12
Dendritic Cells
Apoptosis
MicroRNAs
Monocytes
Cyclin-Dependent Kinase Inhibitor p27
Ubiquitination
human MIRN155 microRNA
Protein Transport
Antigen-Presenting Cells
Hematopoietic Stem Cells
Cell Differentiation
Cytokines

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Immunology
  • Hematology
  • Cell Biology

これを引用

miR-221 and miR-155 regulate human dendritic cell development, apoptosis, and IL-12 production through targeting of p27 kip1, KPC1, and SOCS-1. / Lu, Changming; Huang, Xin; Zhang, Xiaoxiao; Roensch, Kristin; Cao, Qing; Nakayama, Keiichi; Blazar, Bruce R.; Zeng, Yan; Zhou, Xianzheng.

:: Blood, 巻 117, 番号 16, 21.04.2011, p. 4293-4303.

研究成果: ジャーナルへの寄稿記事

Lu, Changming ; Huang, Xin ; Zhang, Xiaoxiao ; Roensch, Kristin ; Cao, Qing ; Nakayama, Keiichi ; Blazar, Bruce R. ; Zeng, Yan ; Zhou, Xianzheng. / miR-221 and miR-155 regulate human dendritic cell development, apoptosis, and IL-12 production through targeting of p27 kip1, KPC1, and SOCS-1. :: Blood. 2011 ; 巻 117, 番号 16. pp. 4293-4303.
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title = "miR-221 and miR-155 regulate human dendritic cell development, apoptosis, and IL-12 production through targeting of p27 kip1, KPC1, and SOCS-1",
abstract = "Dendritic cells (DCs) are potent antigen-presenting cells derived from hematopoietic progenitor cells and circulating monocytes. To investigate the role of microRNAs (miRNAs) during DC differentiation, maturation, and function, we profiled miRNA expression in human monocytes, immature DCs (imDCs), and mature DCs (mDCs). Stage-specific, differential expression of 27 miRNAs was found during monocyte differentiation into imDCs and mDCs. Among them, decreased miR-221 and increased miR-155 expression correlated with p27 kip1 accumulation in DCs. Silencing of miR-221 or overexpressing of miR-155 in DCs resulted in p27 kip1 protein increase and DC apoptosis. Moreover, mDCs from miR-155 -/- mice were less apoptotic than those from wild-type mice. Silencing of miR-155 expression had little effect on DC maturation but reduced IL-12p70 production, whereas miR-155 overexpression in mDCs enhanced IL-12p70 production. Kip1 ubiquitination-promoting complex 1, suppressor of cytokine signaling 1, and CD115 (M-CSFR) were functional targets of miR-155. Furthermore, we provide evidence that miR-155 indirectly regulated p27 kip1 protein level by targeting Kip1 ubiquitination-promoting complex 1. Thus, our study uncovered miRNA signatures during monocyte differentiation into DCs and the new regulatory role of miR-221 and miR-155 in DC apoptosis and IL-12p70 production.",
author = "Changming Lu and Xin Huang and Xiaoxiao Zhang and Kristin Roensch and Qing Cao and Keiichi Nakayama and Blazar, {Bruce R.} and Yan Zeng and Xianzheng Zhou",
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T1 - miR-221 and miR-155 regulate human dendritic cell development, apoptosis, and IL-12 production through targeting of p27 kip1, KPC1, and SOCS-1

AU - Lu, Changming

AU - Huang, Xin

AU - Zhang, Xiaoxiao

AU - Roensch, Kristin

AU - Cao, Qing

AU - Nakayama, Keiichi

AU - Blazar, Bruce R.

AU - Zeng, Yan

AU - Zhou, Xianzheng

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N2 - Dendritic cells (DCs) are potent antigen-presenting cells derived from hematopoietic progenitor cells and circulating monocytes. To investigate the role of microRNAs (miRNAs) during DC differentiation, maturation, and function, we profiled miRNA expression in human monocytes, immature DCs (imDCs), and mature DCs (mDCs). Stage-specific, differential expression of 27 miRNAs was found during monocyte differentiation into imDCs and mDCs. Among them, decreased miR-221 and increased miR-155 expression correlated with p27 kip1 accumulation in DCs. Silencing of miR-221 or overexpressing of miR-155 in DCs resulted in p27 kip1 protein increase and DC apoptosis. Moreover, mDCs from miR-155 -/- mice were less apoptotic than those from wild-type mice. Silencing of miR-155 expression had little effect on DC maturation but reduced IL-12p70 production, whereas miR-155 overexpression in mDCs enhanced IL-12p70 production. Kip1 ubiquitination-promoting complex 1, suppressor of cytokine signaling 1, and CD115 (M-CSFR) were functional targets of miR-155. Furthermore, we provide evidence that miR-155 indirectly regulated p27 kip1 protein level by targeting Kip1 ubiquitination-promoting complex 1. Thus, our study uncovered miRNA signatures during monocyte differentiation into DCs and the new regulatory role of miR-221 and miR-155 in DC apoptosis and IL-12p70 production.

AB - Dendritic cells (DCs) are potent antigen-presenting cells derived from hematopoietic progenitor cells and circulating monocytes. To investigate the role of microRNAs (miRNAs) during DC differentiation, maturation, and function, we profiled miRNA expression in human monocytes, immature DCs (imDCs), and mature DCs (mDCs). Stage-specific, differential expression of 27 miRNAs was found during monocyte differentiation into imDCs and mDCs. Among them, decreased miR-221 and increased miR-155 expression correlated with p27 kip1 accumulation in DCs. Silencing of miR-221 or overexpressing of miR-155 in DCs resulted in p27 kip1 protein increase and DC apoptosis. Moreover, mDCs from miR-155 -/- mice were less apoptotic than those from wild-type mice. Silencing of miR-155 expression had little effect on DC maturation but reduced IL-12p70 production, whereas miR-155 overexpression in mDCs enhanced IL-12p70 production. Kip1 ubiquitination-promoting complex 1, suppressor of cytokine signaling 1, and CD115 (M-CSFR) were functional targets of miR-155. Furthermore, we provide evidence that miR-155 indirectly regulated p27 kip1 protein level by targeting Kip1 ubiquitination-promoting complex 1. Thus, our study uncovered miRNA signatures during monocyte differentiation into DCs and the new regulatory role of miR-221 and miR-155 in DC apoptosis and IL-12p70 production.

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