Mitochondria regulate the differentiation of stem cells from human exfoliated deciduous teeth

Hiroki Kato, Thanh Thi Mai Pham, Haruyoshi Yamaza, Keiji Masuda, Yuta Hirofuji, Xu Han, Hiroshi Sato, Tomoaki Taguchi, Kazuaki Nonaka

研究成果: ジャーナルへの寄稿記事

8 引用 (Scopus)

抄録

Stem cells from human exfoliated deciduous teeth (SHED) are isolated from the dental pulp tissue of primary teeth and can differentiate into neuronal cells. Although SHED are a desirable type of stem cells for transplantation therapy and for the study of neurological diseases, a large part of the neuronal differentiation machinery of SHED remains unclear. Recent studies have suggested that mitochondrial activity is involved in the differentiation of stem cells. In the present work, we investigated the neuronal differentiation machinery of SHED by focusing on mitochondrial activity. During neuronal differentiation of SHED, we observed increased mitochondrial membrane potential, increased mitochondrial DNA, and elongated mitochondria. Furthermore, to examine the demand for mitochondrial activity in neuronal differentiation, we then differentiated SHED into neuronal cells in the presence of rotenone, an inhibitor of mitochondrial respiratory chain complex I, and carbonyl cyanide m-chlorophenyl hydrazone (CCCP), a mitochondrial uncoupler, and found that neuronal differentiation was inhibited by treatment with rotenone and CCCP. These results indicated that increased mitochondrial activity was crucial for the neuronal differentiation of SHED.

元の言語英語
ページ(範囲)105-116
ページ数12
ジャーナルCell structure and function
42
発行部数2
DOI
出版物ステータス出版済み - 1 1 2017

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Carbonyl Cyanide m-Chlorophenyl Hydrazone
Rotenone
Deciduous Tooth
Mitochondria
Stem Cells
Electron Transport Complex I
Dental Pulp
Mitochondrial Membrane Potential
Stem Cell Transplantation
Cell- and Tissue-Based Therapy
Electron Transport
Mitochondrial DNA
Therapeutics

All Science Journal Classification (ASJC) codes

  • Physiology
  • Molecular Biology
  • Cell Biology

これを引用

Mitochondria regulate the differentiation of stem cells from human exfoliated deciduous teeth. / Kato, Hiroki; Pham, Thanh Thi Mai; Yamaza, Haruyoshi; Masuda, Keiji; Hirofuji, Yuta; Han, Xu; Sato, Hiroshi; Taguchi, Tomoaki; Nonaka, Kazuaki.

:: Cell structure and function, 巻 42, 番号 2, 01.01.2017, p. 105-116.

研究成果: ジャーナルへの寄稿記事

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abstract = "Stem cells from human exfoliated deciduous teeth (SHED) are isolated from the dental pulp tissue of primary teeth and can differentiate into neuronal cells. Although SHED are a desirable type of stem cells for transplantation therapy and for the study of neurological diseases, a large part of the neuronal differentiation machinery of SHED remains unclear. Recent studies have suggested that mitochondrial activity is involved in the differentiation of stem cells. In the present work, we investigated the neuronal differentiation machinery of SHED by focusing on mitochondrial activity. During neuronal differentiation of SHED, we observed increased mitochondrial membrane potential, increased mitochondrial DNA, and elongated mitochondria. Furthermore, to examine the demand for mitochondrial activity in neuronal differentiation, we then differentiated SHED into neuronal cells in the presence of rotenone, an inhibitor of mitochondrial respiratory chain complex I, and carbonyl cyanide m-chlorophenyl hydrazone (CCCP), a mitochondrial uncoupler, and found that neuronal differentiation was inhibited by treatment with rotenone and CCCP. These results indicated that increased mitochondrial activity was crucial for the neuronal differentiation of SHED.",
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AU - Pham, Thanh Thi Mai

AU - Yamaza, Haruyoshi

AU - Masuda, Keiji

AU - Hirofuji, Yuta

AU - Han, Xu

AU - Sato, Hiroshi

AU - Taguchi, Tomoaki

AU - Nonaka, Kazuaki

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