Mitochondrial pyruvate carrier 1 expression controls cancer epithelial-mesenchymal transition and radioresistance

Yuji Takaoka, Masamitsu Konno, Jun Koseki, Hugh Colvin, Ayumu Asai, Keisuke Tamari, Taroh Satoh, Masaki Mori, Yuichiro Doki, Kazuhiko Ogawa, Hideshi Ishii

研究成果: ジャーナルへの寄稿記事

3 引用 (Scopus)

抜粋

Mitochondrial pyruvate carrier (MPC) is known to cause different expressions in normal and cancer cells. We observed a change in phenotype with the suppression of MPC expression. We knocked down MPC1 and/or MPC2 using siRNA or shRNA. We observed its cell morphology and accompanying molecular marker. Furthermore, the radioresistance of the MPC knockdown cell line was examined using a colony formation assay. MPC1-suppressed cells changed their morphology to a spindle shape. Epithelial-mesenchymal transition (EMT) was suspected, and examination of the EMT marker by PCR showed a decrease in E-cadherin and an increase in fibronectin. Focusing on glutamine metabolism as the mechanism of this phenomenon, we knocked down the glutamine-metabolizing enzyme glutaminase (GLS). EMT was also observed in GLS-suppressed cells. Furthermore, when MPC1-suppressed cells were cultured in a glutamine-deficient medium, changes in EMT markers were suppressed. In addition, MPC1-suppressed cells also increased with a significant difference in radioresistance. Decreased MPC1 expression favorably affects EMT and radioresistance of cancer.

元の言語英語
ページ(範囲)1331-1339
ページ数9
ジャーナルCancer Science
110
発行部数4
DOI
出版物ステータス出版済み - 4 2019

    フィンガープリント

All Science Journal Classification (ASJC) codes

  • Oncology
  • Cancer Research

これを引用

Takaoka, Y., Konno, M., Koseki, J., Colvin, H., Asai, A., Tamari, K., ... Ishii, H. (2019). Mitochondrial pyruvate carrier 1 expression controls cancer epithelial-mesenchymal transition and radioresistance. Cancer Science, 110(4), 1331-1339. https://doi.org/10.1111/cas.13980