Mitogen-activated protein kinase pathway controls autoimmune regulator (AIRE) gene expression in granulo-monocyte colony stimulating factor (GM-CSF)-stimulated myelomonocytic leukemia OTC-4 cells

Seiho Nagafuchi, Hitoshi Katsuta, Yuju Ohno, Yasushi Inoue, Kazuya Shimoda, Kazuhiko Kogawa, Yukari Ikeda, Rimiko Koyanagi-Katsuta, Satoshi Yamasaki, Hiroyuki Tominaga, Sadafumi Tamiya, Tsukuru Umemura, Takeshi Otsuka, Toshihiro Miyamoto, Teruhisa Otsuka, Mine Harada, Jun Kudoh, Nobuyoshi Shimizu

研究成果: ジャーナルへの寄稿記事

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Autoimmune regulator (AIRE) gene is a responsible gene for the rare autosomal recessive autoimmune disease: autoimmune-polyendocrinopathy- candidiasis ectodermal dystrophy (APECED). Although it has been reported that AIRE is expressed in the thymic epithelial cells and monocyte-dendritic cell lineage, the regulatory mechanisms of AIRE gene expression have as yet been poorly understood. Here we show that the expression of AIRE gene was induced in granulo-monocyte colony stimulating factor (GM-CSF)-stimulated myelomonocytic leukemia OTC-4 cells. In GM-CSF-stimulated OTC-4 cells, stat5 was not phosphorylated, while mitogen-activated protein kinases (MAPKs), including MAPK kinase (MEK) 1/2 and p38 MAPK, were phosphorylated, indicating activation of MAPK pathway. In addition, the expression of AIRE gene was inhibited by specific p38 MAPK inhibitor (SB203580), whereas the expression was rather enhanced by the MEK1/2 inhibitor (U0126), suggesting that AIRE gene expression is regulated by mitogen-activated protein kinase pathway.

元の言語英語
ページ(範囲)130-135
ページ数6
ジャーナルImmunology Letters
99
発行部数1
DOI
出版物ステータス出版済み - 6 15 2005

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All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology

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