Mitogen-induced up-regulation of non-smooth muscle isoform of α- tropomyosin in rat aortic smooth muscle cells

Katsuya Hirano, Mayumi Hirano, Wakako Eto, Junji Nishimura, Hideo Kanaide

研究成果: ジャーナルへの寄稿記事

7 引用 (Scopus)

抄録

Correlation between the expression of the α-tropomyosin isoforms and cell growth was investigated in rat aortic smooth muscle cells. The levels of exon 1a, exons 1a + 2a (smooth muscle type) and exons 1a + 2b (non-smooth muscle type) were determined by reverse transcription-polymerase chain reaction (RT-PCR). When the cells were cultured, the level of exons 1a + 2b transiently increased while reaching a maximum at 3-5 days. When the serum- deprived confluent cells were stimulated with 3-20% serum for 1.5 h, the level of exons 1a + 2b increased by about twofold. The 1-(5- isoquinolinesulphonyl)-2-methylpiperazine (H-7) but not 2-[1-(3- dimethylaminopropyl)-1H-indol-3-yl]-3-(1H-indol-3-yl)-maleimide (GF 109203X) inhibited this up-regulation. Phorbol-12,13-dibutyrate (PDB) mimicked the effect of serum. The DNA synthesis as determined by the incorporation of 5- bromo-2'-deoxy-uridine (BrdU) was not enhanced by the 1.5 h stimulation with serum or phorbol ester. The up-regulation of non-smooth muscle isoform of α- tropomyosin occurred during G0/G1 transition before entering S phase. Protein phosphorylation is suggested to be involved in the up-regulation. However, the responsible kinase(s) remain to be elucidated. (C) 2000 Elsevier Science B.V.

元の言語英語
ページ(範囲)209-218
ページ数10
ジャーナルEuropean Journal of Pharmacology
406
発行部数2
DOI
出版物ステータス出版済み - 10 13 2000

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Tropomyosin
Mitogens
Smooth Muscle Myocytes
Exons
Protein Isoforms
Up-Regulation
Muscles
Serum
Phorbol 12,13-Dibutyrate
1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine
Phorbol Esters
Bromodeoxyuridine
S Phase
Reverse Transcription
Smooth Muscle
Cultured Cells
Phosphotransferases
Phosphorylation
Polymerase Chain Reaction
DNA

All Science Journal Classification (ASJC) codes

  • Pharmacology

これを引用

Mitogen-induced up-regulation of non-smooth muscle isoform of α- tropomyosin in rat aortic smooth muscle cells. / Hirano, Katsuya; Hirano, Mayumi; Eto, Wakako; Nishimura, Junji; Kanaide, Hideo.

:: European Journal of Pharmacology, 巻 406, 番号 2, 13.10.2000, p. 209-218.

研究成果: ジャーナルへの寄稿記事

Hirano, Katsuya ; Hirano, Mayumi ; Eto, Wakako ; Nishimura, Junji ; Kanaide, Hideo. / Mitogen-induced up-regulation of non-smooth muscle isoform of α- tropomyosin in rat aortic smooth muscle cells. :: European Journal of Pharmacology. 2000 ; 巻 406, 番号 2. pp. 209-218.
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