Mitophagy is primarily due to alternative autophagy and requires the MAPK1 and MAPK14 signaling pathways

Yuko Hirota, Shun ichi Yamashita, Yusuke Kurihara, Xiulian Jin, Masamune Aihara, Tetsu Saigusa, Dongchon Kang, Tomotake Kanki

研究成果: ジャーナルへの寄稿記事

53 引用 (Scopus)

抄録

In cultured cells, not many mitochondria are degraded by mitophagy induced by physiological cellular stress. We observed mitophagy in HeLa cells using a method that relies on the pH-sensitive fluorescent protein Keima. With this approach, we found that mitophagy was barely induced by carbonyl cyanide m-chlorophenyl hydrazone treatment, which is widely used as an inducer of PARK2/Parkin-related mitophagy, whereas a small but modest amount of mitochondria were degraded by mitophagy under conditions of starvation or hypoxia. Mitophagy induced by starvation or hypoxia was marginally suppressed by knockdown of ATG7 and ATG12, or MAP1LC3B, which are essential for conventional macroautophagy. In addition, mitophagy was efficiently induced in Atg5 knockout mouse embryonic fibroblasts. However, knockdown of RAB9A and RAB9B, which are essential for alternative autophagy, but not conventional macroautophagy, severely suppressed mitophagy. Finally, we found that the MAPKs MAPK1/ERK2 and MAPK14/p38 were required for mitophagy. Based on these findings, we conclude that mitophagy in mammalian cells predominantly occurs through an alternative autophagy pathway, requiring the MAPK1 and MAPK14 signaling pathways.

元の言語英語
ページ(範囲)332-343
ページ数12
ジャーナルAutophagy
11
発行部数2
DOI
出版物ステータス出版済み - 1 1 2015

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Mitogen-Activated Protein Kinase 14
Mitochondrial Degradation
Autophagy
Starvation
Mitochondria
Carbonyl Cyanide m-Chlorophenyl Hydrazone
HeLa Cells
Knockout Mice
Cultured Cells

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Cell Biology

これを引用

Mitophagy is primarily due to alternative autophagy and requires the MAPK1 and MAPK14 signaling pathways. / Hirota, Yuko; Yamashita, Shun ichi; Kurihara, Yusuke; Jin, Xiulian; Aihara, Masamune; Saigusa, Tetsu; Kang, Dongchon; Kanki, Tomotake.

:: Autophagy, 巻 11, 番号 2, 01.01.2015, p. 332-343.

研究成果: ジャーナルへの寄稿記事

Hirota, Yuko ; Yamashita, Shun ichi ; Kurihara, Yusuke ; Jin, Xiulian ; Aihara, Masamune ; Saigusa, Tetsu ; Kang, Dongchon ; Kanki, Tomotake. / Mitophagy is primarily due to alternative autophagy and requires the MAPK1 and MAPK14 signaling pathways. :: Autophagy. 2015 ; 巻 11, 番号 2. pp. 332-343.
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AU - Aihara, Masamune

AU - Saigusa, Tetsu

AU - Kang, Dongchon

AU - Kanki, Tomotake

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