MNU-induced mutant pools and high performance TILLING enable finding of any gene mutation in rice

Tadzunu Suzuki, Mitsugu Eiguchi, Toshihiro Kumamaru, Hikaru Satoh, Hiroaki Matsusaka, Kazuki Moriguchi, Yasuo Nagato, Nori Kurata

研究成果: ジャーナルへの寄稿記事

132 引用 (Scopus)

抄録

Mutant populations are indispensable genetic resources for functional genomics in all organisms. However, suitable rice mutant populations, induced either by chemicals or irradiation still have been rarely developed to date. To produce mutant pools and to launch a search system for rice gene mutations, we developed mutant populations of Oryza sativa japonica cv. Taichung 65, by treating single zygotic cells with N-methyl-N-nitrosourea (MNU). Mutagenesis in single zygotes can create mutations at a high frequency and rarely forms chimeric plants. A modified TILLING system using non-labeled primers and fast capillary gel electrophoresis was applied for high-throughput detection of single nucleotide substitution mutations. The mutation rate of an M 2 mutant population was calculated as 7.4 × 10 -6 per nucleotide representing one mutation in every 135 kb genome sequence. One can expect 7.4 single nucleotide substitution mutations in every 1 kb of gene region when using 1,000 M 2 mutant lines. The mutations were very evenly distributed over the regions examined. These results indicate that our rice mutant population generated by MNU-mutagenesis could be a promising resource for identifying mutations in any gene of rice. The modified TILLING method also proved very efficient and convenient in screening the mutant population.

元の言語英語
ページ(範囲)213-223
ページ数11
ジャーナルMolecular Genetics and Genomics
279
発行部数3
DOI
出版物ステータス出版済み - 3 1 2008

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Mutation
Genes
Nucleotides
Population
Mutagenesis
Methylnitrosourea
Zygote
Capillary Electrophoresis
Population Genetics
Mutation Rate
Genomics
Oryza
Gels
Genome

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics

これを引用

MNU-induced mutant pools and high performance TILLING enable finding of any gene mutation in rice. / Suzuki, Tadzunu; Eiguchi, Mitsugu; Kumamaru, Toshihiro; Satoh, Hikaru; Matsusaka, Hiroaki; Moriguchi, Kazuki; Nagato, Yasuo; Kurata, Nori.

:: Molecular Genetics and Genomics, 巻 279, 番号 3, 01.03.2008, p. 213-223.

研究成果: ジャーナルへの寄稿記事

Suzuki, T, Eiguchi, M, Kumamaru, T, Satoh, H, Matsusaka, H, Moriguchi, K, Nagato, Y & Kurata, N 2008, 'MNU-induced mutant pools and high performance TILLING enable finding of any gene mutation in rice', Molecular Genetics and Genomics, 巻. 279, 番号 3, pp. 213-223. https://doi.org/10.1007/s00438-007-0293-2
Suzuki, Tadzunu ; Eiguchi, Mitsugu ; Kumamaru, Toshihiro ; Satoh, Hikaru ; Matsusaka, Hiroaki ; Moriguchi, Kazuki ; Nagato, Yasuo ; Kurata, Nori. / MNU-induced mutant pools and high performance TILLING enable finding of any gene mutation in rice. :: Molecular Genetics and Genomics. 2008 ; 巻 279, 番号 3. pp. 213-223.
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