MOB1 regulates thymocyte egress and T-cell survival in mice in a YAP1-independent manner

Wakako Kato, Miki Nishio, Yoko To, Hideru Togashi, Tak Wah Mak, Hidetoshi Takada, Shoichi Ohga, Tomohiko Maehama, Akira Suzuki

研究成果: ジャーナルへの寄稿記事

抄録

Mammalian STE20-like protein kinase 1/2 (MST1/2) and nuclear Dbf2-related kinase 1/2 (NDR1/2) are core components of Hippo signaling that are also known to be important regulators of lymphocyte trafficking. However, little is understood about the roles of other Hippo pathway molecules in these cells. Here, we present the first analysis of the function of Mps one binder kinase activator-1 (MOB1) in T lymphocytes in vivo. T-cell-specific double knockout (DKO) of MOB1A/B in mice [tMob1 DKO mice] reduces the number of naïve T cells in both the circulation and secondary lymphoid organs, but leads to an accumulation of CD4+CD8 and CD4CD8+ single-positive (SP) cells in the thymus. In vitro, naïve MOB1A/B-deficient T cells show increased apoptosis and display impaired trafficking capacity in response to the chemokine CCL19. These defects are linked to suppression of the activation of MST and NDR kinases, but are independent of the downstream transcriptional co-activator Yes-associated protein 1 (YAP1). Thus, MOB1 proteins play an important role in thymic egress and T-cell survival that is mediated by a pathway other than conventional Hippo-YAP1 signaling.

元の言語英語
ページ(範囲)485-495
ページ数11
ジャーナルGenes to Cells
24
発行部数7
DOI
出版物ステータス出版済み - 7 1 2019

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Thymocytes
Cell Survival
Phosphotransferases
T-Lymphocytes
Proteins
Chemokine CCL19
Replication Protein C
Knockout Mice
Protein Kinases
Thymus Gland
Lymphocytes
Apoptosis

All Science Journal Classification (ASJC) codes

  • Genetics
  • Cell Biology

これを引用

Kato, W., Nishio, M., To, Y., Togashi, H., Mak, T. W., Takada, H., ... Suzuki, A. (2019). MOB1 regulates thymocyte egress and T-cell survival in mice in a YAP1-independent manner. Genes to Cells, 24(7), 485-495. https://doi.org/10.1111/gtc.12704

MOB1 regulates thymocyte egress and T-cell survival in mice in a YAP1-independent manner. / Kato, Wakako; Nishio, Miki; To, Yoko; Togashi, Hideru; Mak, Tak Wah; Takada, Hidetoshi; Ohga, Shoichi; Maehama, Tomohiko; Suzuki, Akira.

:: Genes to Cells, 巻 24, 番号 7, 01.07.2019, p. 485-495.

研究成果: ジャーナルへの寄稿記事

Kato, W, Nishio, M, To, Y, Togashi, H, Mak, TW, Takada, H, Ohga, S, Maehama, T & Suzuki, A 2019, 'MOB1 regulates thymocyte egress and T-cell survival in mice in a YAP1-independent manner', Genes to Cells, 巻. 24, 番号 7, pp. 485-495. https://doi.org/10.1111/gtc.12704
Kato W, Nishio M, To Y, Togashi H, Mak TW, Takada H その他. MOB1 regulates thymocyte egress and T-cell survival in mice in a YAP1-independent manner. Genes to Cells. 2019 7 1;24(7):485-495. https://doi.org/10.1111/gtc.12704
Kato, Wakako ; Nishio, Miki ; To, Yoko ; Togashi, Hideru ; Mak, Tak Wah ; Takada, Hidetoshi ; Ohga, Shoichi ; Maehama, Tomohiko ; Suzuki, Akira. / MOB1 regulates thymocyte egress and T-cell survival in mice in a YAP1-independent manner. :: Genes to Cells. 2019 ; 巻 24, 番号 7. pp. 485-495.
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abstract = "Mammalian STE20-like protein kinase 1/2 (MST1/2) and nuclear Dbf2-related kinase 1/2 (NDR1/2) are core components of Hippo signaling that are also known to be important regulators of lymphocyte trafficking. However, little is understood about the roles of other Hippo pathway molecules in these cells. Here, we present the first analysis of the function of Mps one binder kinase activator-1 (MOB1) in T lymphocytes in vivo. T-cell-specific double knockout (DKO) of MOB1A/B in mice [tMob1 DKO mice] reduces the number of na{\"i}ve T cells in both the circulation and secondary lymphoid organs, but leads to an accumulation of CD4+CD8− and CD4−CD8+ single-positive (SP) cells in the thymus. In vitro, na{\"i}ve MOB1A/B-deficient T cells show increased apoptosis and display impaired trafficking capacity in response to the chemokine CCL19. These defects are linked to suppression of the activation of MST and NDR kinases, but are independent of the downstream transcriptional co-activator Yes-associated protein 1 (YAP1). Thus, MOB1 proteins play an important role in thymic egress and T-cell survival that is mediated by a pathway other than conventional Hippo-YAP1 signaling.",
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