Mode of interactions of human aldolase isozymes with cytoskeletons

Takahiro Kusakabe, Kiyohisa Motoki, Katsuji Hori

研究成果: ジャーナルへの寄稿記事

47 引用 (Scopus)

抄録

Three isoforms of fructose-1,6-bisphosphate aldolase were found to bind specifically to the actin-containing filament of the cytoskeleton and to show tissue-specific binding patterns. Aldolase A (muscle type) bound more tightly to the skeletal muscle cytoskeleton among the three isozymes, while aldolase B (liver type) preferred the liver cytoskeleton to those of other tissues. The specific binding of aldolase A to the skeletal muscle eytoskeleton was inhibited strongly by the substrates fructose 1,6-bisphosphate and fructose 1-phosphate. Several mutant aldolases A were examined to identify the amino acid residues or regions that play a role in specific binding. Among the mutant aldolases tested, A-E34D, A-K41N, and A-Y363S exhibited remarkably reduced binding activities. Experiments using FITC-labeled enzymes and Rh- labeled phalloidin disclosed that aldolase A associated with the cytoskeleton. Specifically, when aldolase A was incubated with human fibroblast MRC-5 permeabilized with Triton X-100, aldolase A bound to the actin filaments in the stress fibers within the cell. Aldolase A reversibly inhibited the contraction of MRC-5 cells which usually occurred in the presence of Mg2+-ATP and Ca2+. These results provide direct evidence that aldolase binds specifically to the actin-containing stress fibers and suggest that aldolase may regulate cell contraction through its reversible binding to the filaments in the permeabilized MRC-5 fibroblast.

元の言語英語
ページ(範囲)184-193
ページ数10
ジャーナルArchives of Biochemistry and Biophysics
344
発行部数1
DOI
出版物ステータス出版済み - 8 1 1997
外部発表Yes

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Fructose-Bisphosphate Aldolase
Cytoskeleton
Isoenzymes
Muscle
Actins
Stress Fibers
Fibroblasts
Actin Cytoskeleton
Liver
Skeletal Muscle
Aldehyde-Lyases
Tissue
Phalloidine
Fibers
Fluorescein-5-isothiocyanate
Octoxynol
Protein Isoforms
Adenosine Triphosphate

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology

これを引用

Mode of interactions of human aldolase isozymes with cytoskeletons. / Kusakabe, Takahiro; Motoki, Kiyohisa; Hori, Katsuji.

:: Archives of Biochemistry and Biophysics, 巻 344, 番号 1, 01.08.1997, p. 184-193.

研究成果: ジャーナルへの寄稿記事

Kusakabe, Takahiro ; Motoki, Kiyohisa ; Hori, Katsuji. / Mode of interactions of human aldolase isozymes with cytoskeletons. :: Archives of Biochemistry and Biophysics. 1997 ; 巻 344, 番号 1. pp. 184-193.
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