Modification of fatty acid composition by over- and antisense-expression of a microsomal ω-3 fatty acid desaturase gene in transgenic tobacco

Tatsurou Hamada, Hiroaki Kodama, Mitsuo Nishimura, Koh Iba

研究成果: Contribution to journalArticle査読

20 被引用数 (Scopus)

抄録

ω-3 fatty acid desaturases, which catalyse the conversion of linoleic acid (18:2) to linolenic acid (18:3) in lipids, are located in the microsomes and plastid membranes. Transgenic tobacco plants were produced that express the transcripts of a tobacco microsomal ω-3 fatty acid desaturase gene (NtFad3) in antisense and sense orientations under the control of the cauliflower mosaic virus 35S promoter. The antisease construct has the 0.5-kb fragment of the NtFad3 cDNA containing a 3′-flanking region and a part of the coding region in antisense orientation. The antisense-transformant lines showed decreases of the steady-state NtFad3 mRNA level to 30% of the control plants. In these lines, the 18:3 content decreased to about 80% in root tissues and to about 70-80% in leaf tissues when compared with the control plants. The sense construct has the 1.4-kb full-length cDNA of NtFad3. In one of the sense-transformant lines, the NtFad3 mRNA level increased 8 times when compared with that of the control plants. In this line, the 18:3 content increased by about 1.5-fold in root tissues and by about 1.1-fold in leaf tissues. These results indicate that the up- and down-regulation of the transcript level in the microsomal ω-3 fatty acid desaturase gene is useful to modify the 18:3 content in the vegetative tissues of higher plants.

本文言語英語
ページ(範囲)115-121
ページ数7
ジャーナルTransgenic Research
5
2
DOI
出版ステータス出版済み - 3 1996

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Animal Science and Zoology
  • Agronomy and Crop Science
  • Genetics

フィンガープリント 「Modification of fatty acid composition by over- and antisense-expression of a microsomal ω-3 fatty acid desaturase gene in transgenic tobacco」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル