Modification of ligands for serum albumin on polyethyleneimine to stabilize polyplexes in gene delivery

Yuta Nakamura, Hikari Sato, Takanobu Nobori, Hotaru Matsumoto, Shoko Toyama, Tomohiro Shuno, Akihiro Kishimura, Takeshi Mori, Yoshiki Katayama

研究成果: ジャーナルへの寄稿記事

4 引用 (Scopus)

抄録

In this work, we have developed a new technique to stabilize a ternary complex composed of plasmid DNA, a linear polyethyleneimine (LPEI) and serum albumin. A stearoyl group was conjugated to LPEI as a specific ligand for serum albumin. The resultant ternary complex has excellent stability in physiological saline conditions, maintaining its initial diameter and preventing aggregation of red blood cells. The ternary complex has equivalent transfection ability to and significantly lower cytotoxicity than unmodified LPEI. Therefore, the ternary complex is potentially useful as a new gene carrier, possessing high blood stability.

元の言語英語
ページ(範囲)1382-1393
ページ数12
ジャーナルJournal of Biomaterials Science, Polymer Edition
28
発行部数13
DOI
出版物ステータス出版済み - 9 2 2017

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Polyethyleneimine
Serum Albumin
Blood
Genes
Ligands
Cytotoxicity
DNA
Agglomeration
Cells
Transfection
Plasmids
Erythrocytes

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Bioengineering
  • Biomaterials
  • Biomedical Engineering

これを引用

Modification of ligands for serum albumin on polyethyleneimine to stabilize polyplexes in gene delivery. / Nakamura, Yuta; Sato, Hikari; Nobori, Takanobu; Matsumoto, Hotaru; Toyama, Shoko; Shuno, Tomohiro; Kishimura, Akihiro; Mori, Takeshi; Katayama, Yoshiki.

:: Journal of Biomaterials Science, Polymer Edition, 巻 28, 番号 13, 02.09.2017, p. 1382-1393.

研究成果: ジャーナルへの寄稿記事

Nakamura, Yuta ; Sato, Hikari ; Nobori, Takanobu ; Matsumoto, Hotaru ; Toyama, Shoko ; Shuno, Tomohiro ; Kishimura, Akihiro ; Mori, Takeshi ; Katayama, Yoshiki. / Modification of ligands for serum albumin on polyethyleneimine to stabilize polyplexes in gene delivery. :: Journal of Biomaterials Science, Polymer Edition. 2017 ; 巻 28, 番号 13. pp. 1382-1393.
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