Modification of secondary head-forming activity of microinjected Δβ-catenin mRNA by co-injected spermine and spermidine in Xenopus early embryos

Takamichi Mishina, Kota Fuchimukai, Kazuei Igarashi, Kosuke Tashiro, Koichiro Shiokawa

研究成果: Contribution to journalArticle査読

4 被引用数 (Scopus)

抄録

Polyamines are essential for cell growth and differentiation. In Xenopus early embryos, per embryo level of spermine is extremely low as compared with that of spermidine. To disclose the possible function of polyamines in Xenopus early embryos, we tested the effect of co-injection of spermine and spermidine on the functioning of exogenously microinjected in vitro-synthesized, Δβ-catenin mRNA which is known to induce a secondary head after being microinjected into a ventral vegetal blastomere in 8-celled Xenopus embryos. Microinjection of Δβ-catenin mRNA in fact induced a secondary axis with a secondary head, and co-injection of spermine or spermidine suppresses induction of the secondary head and secondary axis without drastic effects like induction of immediate cell death or execution of apoptosis at blastula stage. The inhibitory effects were dosage dependent, and at lower doses the inhibition was mainly on secondary head formation rather than on secondary axis formation. We performed similar experiments using GFP mRNA and confirmed that expression of GFP mRNA was also suppressed by co-injection of spermine. We mixed Δβ-catenin mRNA with different amounts of spermine and performed electrophoresis on agarose gels, with a finding that the prior mixing greatly suppressed mRNA migration. These results suggest that an excess amount of spermine as well as spermidine exerts inhibitory effects on mRNA translation, and that the inhibition may be due to direct binding of polyamines to mRNA and a reduction of negative charges on mRNA molecules that might also induce the formation of cross link-like networks among mRNAs.

本文言語英語
ページ(範囲)791-801
ページ数11
ジャーナルAmino Acids
42
2-3
DOI
出版ステータス出版済み - 2 2012

All Science Journal Classification (ASJC) codes

  • 生化学
  • 臨床生化学
  • 有機化学

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