Modular surface display for porcine circovirus type 2 virus-like particles mediated by microbial transglutaminase

Akitsu Masuda, Kosuke Minamihata, Masato Hino, Daisuke Morokuma, Noriko Karasaki, Hiroaki Mon, Noriho Kamiya, Takahiro Kusakabe, Man Lee

研究成果: ジャーナルへの寄稿記事

抄録

Virus-like particles (VLPs) are multi-protein complex, which mimic viral particles without viral genomes. Recently various applications of VLPs for medical and pharmaceutical fields are developed. Furthermore, surface modification of VLPs is expected to extend their functional versatility. As an enzymatic strategy for the modification, microbial transglutaminase (MTG) that catalyzes the covalent bond between a glutamine residue and a ly-sine residue or a primary amine is suitable for efficient protein ligation. In the previous study, we demonstrated the efficient production of immunogenic VLPs of porcine circovirus type 2 (PCV2) using silkworm-baculovirus expression vector system (silkworm-BEVS). Herein, we established the display system of exotic molecules to VLPs by enzymatic covalent cross-linking using MTG. For the target modification, Q-tag (YPLQMRG) that is MTG reactive sequence were introduced into the N-terminus, C-terminus, or loop BC of capsid protein of PCV2 and successfully expressed as VLPs in silkworm pupae. Of these, PCV2 VLPs with Q-tag at the loop BC and C-terminus were efficiently conjugated with FITC-cadaverine and K-tagged (MRHKGS) EGFP by MTG. These results showed that flexible surface modification of VLPs mediated by MTG could be used for development of several therapeutic tools such as multivalent vaccines.

元の言語英語
ページ(範囲)53-60
ページ数8
ジャーナルJournal of Insect Biotechnology and Sericology
87
発行部数2
DOI
出版物ステータス出版済み - 1 1 2018

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Circovirus
Porcine circovirus-2
Transglutaminases
virus-like particles
protein-glutamine gamma-glutamyltransferase
Viruses
Virion
Display devices
Bombyx
silkworms
Proteins
Surface treatment
multiprotein complexes
primary amines
Cadaverine
cadaverine
Covalent bonds
Vaccines
Virus
Porcine circovirus

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Applied Microbiology and Biotechnology
  • Business, Management and Accounting(all)
  • Agricultural and Biological Sciences(all)
  • Insect Science
  • Industrial and Manufacturing Engineering

これを引用

Modular surface display for porcine circovirus type 2 virus-like particles mediated by microbial transglutaminase. / Masuda, Akitsu; Minamihata, Kosuke; Hino, Masato; Morokuma, Daisuke; Karasaki, Noriko; Mon, Hiroaki; Kamiya, Noriho; Kusakabe, Takahiro; Lee, Man.

:: Journal of Insect Biotechnology and Sericology, 巻 87, 番号 2, 01.01.2018, p. 53-60.

研究成果: ジャーナルへの寄稿記事

Masuda, Akitsu ; Minamihata, Kosuke ; Hino, Masato ; Morokuma, Daisuke ; Karasaki, Noriko ; Mon, Hiroaki ; Kamiya, Noriho ; Kusakabe, Takahiro ; Lee, Man. / Modular surface display for porcine circovirus type 2 virus-like particles mediated by microbial transglutaminase. :: Journal of Insect Biotechnology and Sericology. 2018 ; 巻 87, 番号 2. pp. 53-60.
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