Molecular analysis and bioactivity of luteinizing hormone from Japanese eel, Anguilla japonica, produced in silkworm pupae

Ji Hyun Choi, Dae Jung Kim, Sun Mee Hong, Sun Jung Jo, Kwan Sik Min, Young Chang Sohn, Jae Man Lee, Takahiro Kusakabe

研究成果: ジャーナルへの寄稿記事

抄録

Luteinizing hormone (LH), a gonadotropin hormone (GTH) of the pituitary glycoprotein family, is important in oocyte maturation, ovulation, and spermiation. In this study, we generated a Japanese eel LH (JeLH) with and without the equine chorionic gonadotropin (eCG) carboxyl-terminal peptides under the control of the polyhedron in the silkworm pupae BES to determine their expression levels, glycosylation profile, and in vitro bioactivity. The target proteins were highly expressed in the pupae hemolymph. Recombinant JeLH·eCG and JeLH were N- or O-glycosylated, as shown by periodic-acid Schiff staining, deglycosidase enzyme treatment, and lectin blot analyses, and showed no significant difference in the in vitro bioactivity. Both single-chain hormones and salmon pituitary extract induced maturation of Japanese eel oocytes in vitro. Recombinant LHs produced in silkworm pupae might be suitable candidates for in vivo experiments, because they can be produced in sufficient amount and can undergo N-glycosylation.

元の言語英語
ページ(範囲)381-388
ページ数8
ジャーナルBiotechnology and Bioprocess Engineering
21
発行部数3
DOI
出版物ステータス出版済み - 6 1 2016

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Anguilla
Glycosylation
Eels
Pupa
Bombyx
Hormones
Luteinizing Hormone
Bioactivity
Pituitary Hormones
Periodic Acid
Oocytes
Chorionic Gonadotropin
Gonadotropins
Lectins
Equine Gonadotropins
Pituitary Gonadotropins
Glycoproteins
Hemolymph
Salmon
Ovulation

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology
  • Biomedical Engineering

これを引用

Molecular analysis and bioactivity of luteinizing hormone from Japanese eel, Anguilla japonica, produced in silkworm pupae. / Choi, Ji Hyun; Kim, Dae Jung; Hong, Sun Mee; Jo, Sun Jung; Min, Kwan Sik; Sohn, Young Chang; Lee, Jae Man; Kusakabe, Takahiro.

:: Biotechnology and Bioprocess Engineering, 巻 21, 番号 3, 01.06.2016, p. 381-388.

研究成果: ジャーナルへの寄稿記事

Choi, Ji Hyun ; Kim, Dae Jung ; Hong, Sun Mee ; Jo, Sun Jung ; Min, Kwan Sik ; Sohn, Young Chang ; Lee, Jae Man ; Kusakabe, Takahiro. / Molecular analysis and bioactivity of luteinizing hormone from Japanese eel, Anguilla japonica, produced in silkworm pupae. :: Biotechnology and Bioprocess Engineering. 2016 ; 巻 21, 番号 3. pp. 381-388.
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abstract = "Luteinizing hormone (LH), a gonadotropin hormone (GTH) of the pituitary glycoprotein family, is important in oocyte maturation, ovulation, and spermiation. In this study, we generated a Japanese eel LH (JeLH) with and without the equine chorionic gonadotropin (eCG) carboxyl-terminal peptides under the control of the polyhedron in the silkworm pupae BES to determine their expression levels, glycosylation profile, and in vitro bioactivity. The target proteins were highly expressed in the pupae hemolymph. Recombinant JeLH·eCG and JeLH were N- or O-glycosylated, as shown by periodic-acid Schiff staining, deglycosidase enzyme treatment, and lectin blot analyses, and showed no significant difference in the in vitro bioactivity. Both single-chain hormones and salmon pituitary extract induced maturation of Japanese eel oocytes in vitro. Recombinant LHs produced in silkworm pupae might be suitable candidates for in vivo experiments, because they can be produced in sufficient amount and can undergo N-glycosylation.",
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AU - Jo, Sun Jung

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AU - Sohn, Young Chang

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AU - Kusakabe, Takahiro

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