Molecular analysis of fungal populations in patients with oral candidiasis using next-generation sequencing

Yumi Imabayashi, Masafumi Moriyama, Toru Takeshita, Shinsuke Ieda, Jun Nosuke Hayashida, Akihiko Tanaka, Takashi Maehara, Sachiko Furukawa, Miho Ohta, Keigo Kubota, Masaki Yamauchi, Noriko Ishiguro, Yoshihisa Yamashita, Seiji Nakamura

研究成果: Contribution to journalArticle査読

10 被引用数 (Scopus)

抄録

Oral candidiasis is closely associated with changes in oral fungal biodiversity and is caused primarily by Candida albicans. However, the widespread use of empiric and prophylactic antifungal drugs has caused a shift in fungal biodiversity towards other Candida or yeast species. Recently, next-generation sequencing (NGS) has provided an improvement over conventional culture techniques, allowing rapid comprehensive analysis of oral fungal biodiversity. In this study, we used NGS to examine the oral fungal biodiversity of 27 patients with pseudomembranous oral candidiasis (POC) and 66 healthy controls. The total number of fungal species in patients with POC and healthy controls was 67 and 86, respectively. The copy number of total PCR products and the proportion of non-C. albicans, especially C. dubliniensis, in patients with POC, were higher than those in healthy controls. The detection patterns in patients with POC were similar to those in controls after antifungal treatment. Interestingly, the number of fungal species and the copy number of total PCR products in healthy controls increased with aging. These results suggest that high fungal biodiversity and aging might be involved in the pathogenesis of oral candidiasis. We therefore conclude that NGS is a useful technique for investigating oral candida infections.

本文言語英語
論文番号28110
ジャーナルScientific reports
6
DOI
出版ステータス出版済み - 6 16 2016

All Science Journal Classification (ASJC) codes

  • 一般

フィンガープリント

「Molecular analysis of fungal populations in patients with oral candidiasis using next-generation sequencing」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル