Molecular analysis of fungal populations in patients with oral candidiasis using next-generation sequencing

Yumi Imabayashi, Masafumi Moriyama, Toru Takeshita, Shinsuke Ieda, Hayashida Jun-Nosuke, Akihiko Tanaka, takashi maehara, Sachiko Furukawa, Miho Ohta, Keigo Kubota, Masaki Yamauchi, Noriko Ishiguro, Yoshihisa Yamashita, Seiji Nakamura

研究成果: ジャーナルへの寄稿記事

8 引用 (Scopus)

抄録

Oral candidiasis is closely associated with changes in oral fungal biodiversity and is caused primarily by Candida albicans. However, the widespread use of empiric and prophylactic antifungal drugs has caused a shift in fungal biodiversity towards other Candida or yeast species. Recently, next-generation sequencing (NGS) has provided an improvement over conventional culture techniques, allowing rapid comprehensive analysis of oral fungal biodiversity. In this study, we used NGS to examine the oral fungal biodiversity of 27 patients with pseudomembranous oral candidiasis (POC) and 66 healthy controls. The total number of fungal species in patients with POC and healthy controls was 67 and 86, respectively. The copy number of total PCR products and the proportion of non-C. albicans, especially C. dubliniensis, in patients with POC, were higher than those in healthy controls. The detection patterns in patients with POC were similar to those in controls after antifungal treatment. Interestingly, the number of fungal species and the copy number of total PCR products in healthy controls increased with aging. These results suggest that high fungal biodiversity and aging might be involved in the pathogenesis of oral candidiasis. We therefore conclude that NGS is a useful technique for investigating oral candida infections.

元の言語英語
記事番号28110
ジャーナルScientific reports
6
DOI
出版物ステータス出版済み - 6 16 2016

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Oral Candidiasis
Biodiversity
Population
Candida
Culture Techniques
Polymerase Chain Reaction
Candida albicans
Yeasts
Infection
Pharmaceutical Preparations

All Science Journal Classification (ASJC) codes

  • General

これを引用

Molecular analysis of fungal populations in patients with oral candidiasis using next-generation sequencing. / Imabayashi, Yumi; Moriyama, Masafumi; Takeshita, Toru; Ieda, Shinsuke; Jun-Nosuke, Hayashida; Tanaka, Akihiko; maehara, takashi; Furukawa, Sachiko; Ohta, Miho; Kubota, Keigo; Yamauchi, Masaki; Ishiguro, Noriko; Yamashita, Yoshihisa; Nakamura, Seiji.

:: Scientific reports, 巻 6, 28110, 16.06.2016.

研究成果: ジャーナルへの寄稿記事

Imabayashi, Yumi ; Moriyama, Masafumi ; Takeshita, Toru ; Ieda, Shinsuke ; Jun-Nosuke, Hayashida ; Tanaka, Akihiko ; maehara, takashi ; Furukawa, Sachiko ; Ohta, Miho ; Kubota, Keigo ; Yamauchi, Masaki ; Ishiguro, Noriko ; Yamashita, Yoshihisa ; Nakamura, Seiji. / Molecular analysis of fungal populations in patients with oral candidiasis using next-generation sequencing. :: Scientific reports. 2016 ; 巻 6.
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abstract = "Oral candidiasis is closely associated with changes in oral fungal biodiversity and is caused primarily by Candida albicans. However, the widespread use of empiric and prophylactic antifungal drugs has caused a shift in fungal biodiversity towards other Candida or yeast species. Recently, next-generation sequencing (NGS) has provided an improvement over conventional culture techniques, allowing rapid comprehensive analysis of oral fungal biodiversity. In this study, we used NGS to examine the oral fungal biodiversity of 27 patients with pseudomembranous oral candidiasis (POC) and 66 healthy controls. The total number of fungal species in patients with POC and healthy controls was 67 and 86, respectively. The copy number of total PCR products and the proportion of non-C. albicans, especially C. dubliniensis, in patients with POC, were higher than those in healthy controls. The detection patterns in patients with POC were similar to those in controls after antifungal treatment. Interestingly, the number of fungal species and the copy number of total PCR products in healthy controls increased with aging. These results suggest that high fungal biodiversity and aging might be involved in the pathogenesis of oral candidiasis. We therefore conclude that NGS is a useful technique for investigating oral candida infections.",
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