Molecular analysis of the folC gene of Pseudomonas aeruginosa

Takahiro Murata, Andrew L. Bognar, Tetsuya Hayashi, Makoto Ohnishi, Keisuke Nakayama, Yoshiro Terawaki

研究成果: ジャーナルへの寄稿記事

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We have cloned the Pseudomonas aeruginosa folC gene coding for folylpolyglutamate synthetase-dihydrofolate synthetase, which was located between the trpF and purF loci, and determined the nucleotide sequence of the folC gene and its flanking region. The deduced amino acid sequence of P. aeruginosa FolC was highly homologous to that of Escherichia coli FolC. The cloned gene complemented E. coli folC mutations and was found to encode both folylpolyglutamate synthetase and dihydrofolate synthetase activities. The gene organization around the folC gene in P. aeruginosa was completely conserved with that in E. coli; the accD gene was located upstream of the folC gene, and dedD, cvpA and purF genes followed the folC gene in this order. The gene arrangement and the result of the promoter activity assay suggested that the P. aeruginosa accD and folC genes were co-transcribed.

元の言語英語
ページ(範囲)879-886
ページ数8
ジャーナルMICROBIOLOGY and IMMUNOLOGY
44
発行部数11
DOI
出版物ステータス出版済み - 1 1 2000
外部発表Yes

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All Science Journal Classification (ASJC) codes

  • Microbiology
  • Immunology
  • Virology

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