Molecular and biochemical characterization of a Zeta-class glutathione S-transferase of the silkmoth

Kohji Yamamoto, Yuichi Shigeoka, Yoichi Aso, Yutaka Banno, Makoto Kimura, Takashi Nakashima

研究成果: Contribution to journalArticle査読

56 被引用数 (Scopus)

抄録

A cDNA encoding Zeta-class GST of the silkmoth, Bombyx mori (bmGSTZ), was cloned by a reverse transcriptase-polymerase chain reaction. The resulting clone was sequenced and deduced for amino acid sequence, which revealed 45-50% identities to Zeta-class GSTs from other organisms. A recombinant protein (rbmGSTZ) was functionally overexpressed in Bombyx mori cells in a soluble form and purified to homogeneity. rbmGSTZ was able to catalyze the biotranslation of glutathione with dichloroacetic acid. We found that the present GST-catalyzed dechlorination of permethrin and distributed abundantly in silkmoth strain exhibiting permethrin resistance. Our results suggest that bmGSTZ could contribute to permethrin resistance in lepidopteran.

本文言語英語
ページ(範囲)30-35
ページ数6
ジャーナルPesticide Biochemistry and Physiology
94
1
DOI
出版ステータス出版済み - 5 2009

All Science Journal Classification (ASJC) codes

  • 農業および作物学
  • 健康、毒物学および変異誘発

フィンガープリント

「Molecular and biochemical characterization of a Zeta-class glutathione S-transferase of the silkmoth」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル