Molecular and biochemical characterization of manganese-containing superoxide dismutase from the silkworm, Bombyx mori

Kohji Yamamoto, Pingbo Zhang, Ningjia He, Yongqiang Wang, Yoichi Aso, Yutaka Banno, Hiroshi Fujii

研究成果: ジャーナルへの寄稿記事

16 引用 (Scopus)

抄録

Superoxide dismutase (SOD) is responsible for the removal of superoxide anion from living organisms. In this study, cDNA encoding the manganese-containing SOD (MnSOD) from the silkworm, Bombyx mori, was isolated by reverse transcriptase-polymerase chain reaction and sequenced. The deduced amino acid sequence of the MnSOD revealed 62% identity to that of the Drosophila melanogaster; both were close to each other in a phylogenetic tree. The MnSOD was overproduced in Escherichia coli and purified. The internal structure of the recombinant MnSOD was confirmed by peptide mass fingerprinting method. The recombinant MnSOD facilitating the reduction reaction of superoxide anion retained 75% of its original activity after incubation at pH 4-11 for 24 h at 4°C. Its activity was never affected by incubation at pH 7 for 30 min below 50°C.

元の言語英語
ページ(範囲)403-409
ページ数7
ジャーナルComparative Biochemistry and Physiology - B Biochemistry and Molecular Biology
142
発行部数4
DOI
出版物ステータス出版済み - 12 1 2005

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Bombyx
Manganese
Superoxide Dismutase
Superoxides
Peptide Mapping
Drosophila melanogaster
Reverse Transcriptase Polymerase Chain Reaction
Polymerase chain reaction
RNA-Directed DNA Polymerase
Amino Acid Sequence
Complementary DNA
Escherichia coli
Amino Acids
Peptides

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Physiology
  • Molecular Biology

これを引用

Molecular and biochemical characterization of manganese-containing superoxide dismutase from the silkworm, Bombyx mori. / Yamamoto, Kohji; Zhang, Pingbo; He, Ningjia; Wang, Yongqiang; Aso, Yoichi; Banno, Yutaka; Fujii, Hiroshi.

:: Comparative Biochemistry and Physiology - B Biochemistry and Molecular Biology, 巻 142, 番号 4, 01.12.2005, p. 403-409.

研究成果: ジャーナルへの寄稿記事

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