Molecular and biophysical properties of voltage-gated Na+ channels in murine vas deferens

Hai Lei Zhu, Manami Aishima, Hidetaka Morinaga, Richard D. Wassall, Atsushi Shibata, Kazuomi Iwasa, Masatoshi Nomura, Masaya Nagao, Katsuo Sueishi, Thomas C. Cunnane, Noriyoshi Teramoto

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The biological and molecular properties of tetrodotoxin (TTX)-sensitive voltage-gated Na+ currents (INa) in murine vas deferens myocytes were investigated using patch-clamp techniques and molecular biological analyses. In whole-cell configuration, a fast, transient inward current was evoked in the presence of Cd2+, and was abolished by TTX (K d = 11.2 nM), mibefradil (Kd=3.3 μM), and external replacement of Na+ with monovalent cations (TEA+, Tris+, and NMDG+). The fast transient inward current was enhanced by veratridine, an activator of voltage-gated Na+ channels, suggesting that the fast transient inward current was a TTX-sensitive I Na. The values for half-maximal (Vhalf) inactivation and activation of INa were -46.3 mV and -26.0 mV, respectively. RT-PCR analysis revealed the expression of Scn1a, 2a, and 8a transcripts. The Scn8a transcript and the α-subunit protein of NaV1.6 were detected in smooth muscle layers. Using NaV1.6-null mice (NaV1.6 -/-) lacking the expression of the Na+ channel gene, Scn8a, INa were not detected in dispersed smooth muscle cells from the vas deferens, while TTX-sensitive INa were recorded in their wild-type (NaV1.6+/+) littermates. This study demonstrates that the molecular identity of the voltage-gated Na+ channels responsible for the TTX-sensitive INa in murine vas deferens myocytes is primarily NaV1.6.

元の言語英語
ページ(範囲)3340-3351
ページ数12
ジャーナルBiophysical Journal
94
発行部数8
DOI
出版物ステータス出版済み - 4 15 2008

All Science Journal Classification (ASJC) codes

  • Biophysics

フィンガープリント Molecular and biophysical properties of voltage-gated Na<sup>+</sup> channels in murine vas deferens' の研究トピックを掘り下げます。これらはともに一意のフィンガープリントを構成します。

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    Zhu, H. L., Aishima, M., Morinaga, H., Wassall, R. D., Shibata, A., Iwasa, K., Nomura, M., Nagao, M., Sueishi, K., Cunnane, T. C., & Teramoto, N. (2008). Molecular and biophysical properties of voltage-gated Na+ channels in murine vas deferens. Biophysical Journal, 94(8), 3340-3351. https://doi.org/10.1529/biophysj.107.117192