Molecular and immunological characterization of a 64‐kDa protein of Actinobacillus actinomycetemcomitans

Y. Nakano, Yuuko Inai, Yoshihisa Yamashita, S. Nagaoka, T. Kusuzaki‐Nagira, T. Nishihara, N. Okahashi, T. Koga

研究成果: ジャーナルへの寄稿記事

26 引用 (Scopus)

抄録

The 64‐kDa protein to which about half the sera from patients with localized juvenile periodontitis and rapidly progressive periodontitis reacted strongly was purified from Actinobacillus actinomycetemcomitans Y4. Determination of the TV‐terminal sequence of the protein revealed that it was a GroEL‐like protein. The DNA fragment containing the groEL gene of A. actinomycetemcomitans was amplified by polymerase chain reaction, and the groESL operon was cloned by using colony hybridization with the amplified fragment from A. actinomycetemcomitans chromosomal DNA. Sequence analysis revealed that structures of the operon and its products were typical in gram‐negative bacteria. Rabbit polyclonal antibodies to the 64‐kDa protein cross‐reacted with approximately 65‐kDa proteins of Haemophilus aphrophilus, Haemophilus influenzae, Haemophilus paraphrophilus, Escherichia coli and Eikenella corrodens but not with any cellular proteins of Porphyromonas gingivalis, Prevotella intermedia and Fusobacteriuni nucleatum. It is possible that antibodies reactive to the 64‐kDa protein in periodontitis patients are induced by the cross‐reactivity with the hsp60 proteins of other bacteria.

元の言語英語
ページ(範囲)151-159
ページ数9
ジャーナルOral Microbiology and Immunology
10
発行部数3
DOI
出版物ステータス出版済み - 1 1 1995

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Aggregatibacter actinomycetemcomitans
Proteins
Periodontitis
Operon
Haemophilus paraphrophilus
Aggregatibacter aphrophilus
Eikenella corrodens
Prevotella intermedia
Chaperonin 60
Aggressive Periodontitis
Bacteria
Porphyromonas gingivalis
Antibodies
DNA
Protein Sequence Analysis
Haemophilus influenzae
Sequence Analysis
Escherichia coli
Rabbits
Polymerase Chain Reaction

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Immunology
  • Dentistry(all)
  • Microbiology (medical)

これを引用

Molecular and immunological characterization of a 64‐kDa protein of Actinobacillus actinomycetemcomitans. / Nakano, Y.; Inai, Yuuko; Yamashita, Yoshihisa; Nagaoka, S.; Kusuzaki‐Nagira, T.; Nishihara, T.; Okahashi, N.; Koga, T.

:: Oral Microbiology and Immunology, 巻 10, 番号 3, 01.01.1995, p. 151-159.

研究成果: ジャーナルへの寄稿記事

Nakano, Y. ; Inai, Yuuko ; Yamashita, Yoshihisa ; Nagaoka, S. ; Kusuzaki‐Nagira, T. ; Nishihara, T. ; Okahashi, N. ; Koga, T. / Molecular and immunological characterization of a 64‐kDa protein of Actinobacillus actinomycetemcomitans. :: Oral Microbiology and Immunology. 1995 ; 巻 10, 番号 3. pp. 151-159.
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AU - Kusuzaki‐Nagira, T.

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