Molecular bases for human complement C7 polymorphisms, C7*3 and C7*4

Takahiko Horiuchi, Hiroaki Nishimukai, Tatsuyuki Okiura, Koji Nishimura, Hiroaki Nishizaka, Takeshi Kojima, Hiroshi Tsukamoto, Kenshi Hayashi, Mine Harada

研究成果: Contribution to journalArticle査読

5 被引用数 (Scopus)

抄録

Complement C7 is one of the components of membrane attack complex (MAC) generated by the terminal complement cascade. C7 protein is polymorphic and most of its polymorphisms have been identified using isoelectric focusing (IEF), which detects protein charge differences. To date, the molecular bases of the polymorphisms detected by IEF have not been determined. In this paper, we describe the structural bases of two C7 IEF-detected polymorphisms, C7*3 and C7*4, both of which are common in Asian populations. C7*3 resulted from substitution of cysteine (Cys) at amino acid residue 106 by charged arginine (Arg; C106R), while charged lysine (Lys) at amino acid residue 398 was replaced by neutral glutamine (Gln; K398Q) in C7*4. As C7*3 is hypomorphic, it is important to study its possible associations with diseases such as immunological disorders and infections. We present genetic bases for this C7 polymorphism, which we determined using polymerase chain reaction (PCR)-based genotyping, a simple and accurate method suitable for large-scale studies.

本文言語英語
ページ(範囲)450-455
ページ数6
ジャーナルBiochemical and Biophysical Research Communications
298
3
DOI
出版ステータス出版済み - 2002

All Science Journal Classification (ASJC) codes

  • 生物理学
  • 生化学
  • 分子生物学
  • 細胞生物学

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